We narrowed to 10,636 results for: t7

1. pPSU20-40-60-80

   Plasmid

   #173839

   Purpose

   Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACCBPv3
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK225

   Plasmid

   #71124

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet-1_Ec_rpoZ

   Plasmid

   #128837

   Purpose

   E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

   Depositor

   Seth Darst

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Insert

   rpoZ (rpoZ E. coli)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET A-mEosEM-E

   Plasmid

   #226336

   Purpose

   Photoswitchable fluorescent protein for correlative light-electron microscopy; mEosEM-E for prokaryotic expression

   Depositor

   Pingyong Xu

   Article

   Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

   Insert

   mEosEM-E
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKAR2-Br512

   Plasmid

   #161875

   Purpose

   Expresses Br512 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Maranhao et al medRxiv

   Insert

   Modified DNA Polymerase I Large Fragment (Br512)
   

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Promoter

   T7, tetPA

   Available Since

   Nov. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. OaAEP1_C247A

   Plasmid

   #200307

   Purpose

   Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

   Depositor

   Artur Krężel

   Article

   Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

   Insert

   Asparaginyl endopeptidase from Oldenlandia affinis
   

   Tags

   Smt3

   Expression

   Bacterial

   Mutation

   Cysteine 247 to Alanine

   Promoter

   T7

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b Hot-PETase

   Plasmid

   #225761

   Purpose

   Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   Hot-PETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYFJ84

   Plasmid

   #227644

   Purpose

   Vibrio harveyi acyl-ACP synthetase

   Depositor

   John Cronan

   Article

   Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

   Insert

   Acyl-Acyl carrier protein synthetase
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-TEV-SNAP-PRC1

   Plasmid

   #167541

   Purpose

   For expression of His6-TEV-SNAP-PRC1 in bacteria.

   Depositor

   Tarun Kapoor

   Article

   Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

   Insert

   PRC1 (PRC1 Human)
   

   Tags

   His6 tag and SNAP tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL-MHCK7-GCaMP6

    Plasmid

    #65042

    Purpose

    Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

    Depositor

    Nenad Bursac

    Article

    Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

    Insert

    GCaMP6
    

    Use

    Lentiviral

    Tags

    6xHis, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Promoter

    MHCK7

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9-polyA

    Plasmid

    #72602

    Purpose

    express hCas9 byT7 promoter with polyadenine tails

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

    Insert

    Cas9-polyadenine
    

    Tags

    81-bp polyadenylation

    Promoter

    T7

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDae029 (eMutaT7[TadA-8e])

    Plasmid

    #187620

    Purpose

    Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    TadA-8e-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPYtag-Histag-Avidin

    Plasmid

    #214836

    Purpose

    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    SPYtag-Histag-Avidin
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24b-APEX2

    Plasmid

    #111702

    Purpose

    For IPTG-inducible expression of APEX2 in E. coli

    Depositor

    Jessica Seeliger

    Article

    Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

    Insert

    APEX2
    

    Tags

    6xHis and V5

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTXTL-T7max-HiBiTGFPHis

    Plasmid

    #188317

    Purpose

    Expression of HiBiT-GFP via T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    HiBiT-GFP fusion
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKDC.109

    Plasmid

    #227189

    Purpose

    T7 IPTG-driven Cas9

    Depositor

    Seth Shipman

    Article

    Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

    Insert

    SpyCas9
    

    Expression

    Bacterial

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET24_NusA-Tus

    Plasmid

    #165959

    Purpose

    Expression of soluble E. coli Tus protein

    Depositor

    Keith Pardee

    Article

    Norouzi et al ACS Synth Biol. 2021 Jun 23. doi: 10.102

    Insert

    Tus
    

    Tags

    His-NusA-Tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET22b RNase A

    Plasmid

    #58903

    Purpose

    Bacterial expression of bovine pancreatic ribonuclease A

    Depositor

    Ronald Raines

    Article

    delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

    Insert

    RNase A (RNASE1 Bovine)
    

    Tags

    pelB sequence

    Expression

    Bacterial

    Mutation

    See Depositor Comments below

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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