We narrowed to 10,636 results for: t7

1. His6TEV-UbG76C

   Plasmid

   #75299

   Purpose

   Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

   Depositor

   Cynthia Wolberger

   Article

   Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

   Insert

   human Ubiquitin mutant G76 to C
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Glycine 76 to Cystine

   Promoter

   T7

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET20b- hTPI

   Plasmid

   #50723

   Purpose

   overexpression of human TPI1 in E.coli

   Depositor

   Markus Ralser

   Article

   Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

   Insert

   Triosephosphate isomerase 1 (TPI1 Human)
   

   Tags

   6xHIS tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. CP2-TCC

   Plasmid

   #179679

   Purpose

   Complementary plasmid

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [sd2] T7 RNAP-TCC linker-degron
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPdR

   Plasmid

   #85083

   Purpose

   Expresses putidaredoxin reductase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin reductase
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Non tagged Avidin

   Plasmid

   #214835

   Purpose

   This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

   Insert

   Non tagged Avidin
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETA_crE6

   Plasmid

   #172928

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkers

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-SpyCatcher002

   Plasmid

   #102827

   Purpose

   Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. luxB:cp157Venus+luxA/pRSETB Duet

   Plasmid

   #197583

   Purpose

   BRET lux luciferase with cp157Venus for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxBcp157Venus
   

   Use

   E.coli expression

   Tags

   His X 6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_T7-ARG1

   Plasmid

   #106473

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. RpHLuorin2

    Plasmid

    #180227

    Purpose

    for bacterial expression of recombinant ratiometric pHluorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2
    

    Tags

    His-tag followed by thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. hUbc13

    Plasmid

    #51131

    Purpose

    human ubc13, E2 for synthesis k63 linked ubiquitin chain

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    ubc13 (UBE2N Human)
    

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pGEM4Z-EGFP

    Plasmid

    #183475

    Purpose

    This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    EGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CeNL_pRSETb

    Plasmid

    #89510

    Purpose

    Cyan color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Tags

    His X 6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Neq2X7

    Plasmid

    #182366

    Purpose

    Expresses Neq2X7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X7 DNA polymerase
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a-hPKM1

    Plasmid

    #44241

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M1 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. HTATIP (2OU2)

    Plasmid

    #33338

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HTATIP (KAT5 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pET11a+CYP119

    Plasmid

    #66131

    Purpose

    Expresses the wild type of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-At-TRL

    Plasmid

    #32242

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    A. thaliana tRNA ligase (RNL Mustard Weed)
    

    Tags

    6xHistidine

    Expression

    Bacterial

    Promoter

    T7 RNA Polmyerase

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

    Plasmid

    #231128

    Purpose

    Expresses xylanase via a cSAT2.0 scheme

    Depositor

    Xiaofeng Yang

    Article

    Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

    Insert

    xylanase
    

    Tags

    cSAT2.0

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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