We narrowed to 10,636 results for: t7

1. pRM006

   Plasmid

   #98922

   Purpose

   Express Histag-SUMO-FtsZ for purification in E coli

   Depositor

   Jie Xiao

   Article

   Yang et al Science. 2017 Feb 17;355(6326):744-747.

   Insert

   FtsZ
   

   Tags

   Histag-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-Simple-gRNA backbone

   Plasmid

   #51306

   Purpose

   gRNA expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Type

   Empty backbone

   Use

   CRISPR; Xenopus expression

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJExpress414_GmPOPB-S577A

   Plasmid

   #92235

   Purpose

   Express S577A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-S577A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Serine 577 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJExpress414_GmPOPB-D661A

   Plasmid

   #92236

   Purpose

   Express D661A mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-D661A mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Aspartate 661 to Alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJExpress414_GmPOPB-H698N

   Plasmid

   #92242

   Purpose

   Express H698N mutant of prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata-H698N mutant
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Histidine 698 to Asparagine

   Promoter

   T7 promoter

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EcRho

   Plasmid

   #113121

   Purpose

   Expresses E. coli Rho

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   Rho
   

   Tags

   MGH

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet1_CoiC_CoiSA

   Plasmid

   #208762

   Purpose

   Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiC
   CoiSA
   

   Expression

   Bacterial

   Mutation

   single nucleotide insertion (C70) at RBS of CoiC

   Promoter

   T7

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM236 Ubc4

   Plasmid

   #226361

   Purpose

   Expression of yeast Ubc4

   Depositor

   Andreas Martin

   Article

   Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

   Insert

   Ubc4 (UBC4 Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PBAD-his6-prkA-pACYC184

   Plasmid

   #41041

   Depositor

   Ichiro Matsumura

   Article

   Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

   Insert

   prkA
   

   Tags

   6xHis and T7 epitope tag

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-OTI-TCRA_p2_TCRB

    Plasmid

    #131033

    Purpose

    Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

    Depositor

    Jeff Hammerbacher

    Article

    Aksoy et al bioRxiv

    Inserts

    TCRA (Tcra Mouse)
    TCRB (Tcrb Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV/T7

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPH6

    Plasmid

    #40315

    Depositor

    Shaun Lott

    Article

    Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

    Insert

    Peptide:N-glycosidase F
    

    Tags

    6xHis and OmpA3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-LSSmCherry1

    Plasmid

    #89986

    Purpose

    Long stokes-shift variant of red fluorescent protein mCherry

    Depositor

    Robert Campbell

    Article

    Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

    Insert

    LSSmCherry1
    

    Tags

    6xHis-T7-Xpress

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SUPREM

    Plasmid

    #205023

    Purpose

    Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    SUPer Rna Ecogii Methyltransferase
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a-hPKM2

    Plasmid

    #44242

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST14-DoubleCatcher

    Plasmid

    #216284

    Purpose

    Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAT-HA

    Plasmid

    #35612

    Depositor

    Steven Dowdy

    Article

    Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

    Type

    Empty backbone

    Tags

    6xHIS, HA, and TAT

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALOD4

    Plasmid

    #111026

    Purpose

    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A

    Promoter

    T7

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. nifF in pET21b

    Plasmid

    #124520

    Purpose

    Azotobacter vinelandii flavodoxin II of Nitrogenase

    Depositor

    Douglas Rees

    Article

    Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

    Insert

    Flavodoxin II (nifF Azotobacter vinelandii)
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    T7 / lac

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-TEV-PRC1

    Plasmid

    #167539

    Purpose

    For expression of His6-TEV-PRC1 in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

    Insert

    PRC1 (PRC1 Human)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC_MS2_DNA_VLP

    Plasmid

    #179156

    Purpose

    Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

    Depositor

    Paul Freemont

    Article

    Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

    Inserts

    Maturation Protein
    Coat Protein Dimer
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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