We narrowed to 10,636 results for: t7

1. pE5770

   Plasmid

   #170777

   Purpose

   expression of hybrid proteins in E. coli

   Depositor

   Anthony Schryvers

   Article

   Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

   Insert

   Kan resistance (kanR )
   

   Tags

   polyhisbioMbpTEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCrhaB2plus

   Plasmid

   #164226

   Purpose

   Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

   Depositor

   Silvia Cardona

   Article

   Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Rhamnose-inducible

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCSG7-Wt-NmeCas9

   Plasmid

   #71474

   Purpose

   bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-WT-NmeCas9
   

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIS1_Opt_Renilla

   Plasmid

   #210571

   Purpose

   Expresses Optimal Renilla luciferase

   Depositor

   Olivia Rissland

   Article

   Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

   Insert

   Optimal Renilla
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   HSV TK; T7

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11a-SUMO3

   Plasmid

   #53143

   Purpose

   E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   SUMO3 (SUMO2 Human)
   

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-93)

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11b-HA2

   Plasmid

   #165992

   Purpose

   Influenza HA ministem with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   HA2
   

   Tags

   Foldon and HisTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pChuA

   Plasmid

   #42539

   Depositor

   Alan Jasanoff

   Article

   Lelyveld et al J Am Chem Soc. 2011 Feb 2;133(4):649-51.

   Insert

   ChuA (chuA E. coli O157:H7)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET22b-6h-GST-TEVp

   Plasmid

   #172887

   Purpose

   Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Insert

   TEV protease
   

   Tags

   GST solubility tag and HHHHHH

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET11a-SUMO2

   Plasmid

   #53142

   Purpose

   E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   SUMO2 (SUMO3 Human)
   

   Expression

   Bacterial

   Mutation

   mature form (amino acids 1-92)

   Promoter

   T7

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. enz.099-PKA

    Plasmid

    #154048

    Purpose

    Expresses PKA in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Protein kinase cAMP-activated catalytic subunit alpha (PKA) (PRKACA Human)
    

    Tags

    His, MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. His-TEV-EGFP-PRC1

    Plasmid

    #167540

    Purpose

    For expression of His6-TEV-EGFP-PRC1 in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

    Insert

    PRC1 (PRC1 Human)
    

    Tags

    EGFP and His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SnoopTagJr-MBP

    Plasmid

    #105628

    Purpose

    Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SnoopTagJr-MBP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET29b Tau Cys

    Plasmid

    #108867

    Purpose

    Expresses human Tau with a mutated cysteine

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Expression

    Bacterial

    Mutation

    I260C C291A C322A to allow maleimide dye labelling

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Ran-T24N-mRFP1-polyA

    Plasmid

    #104561

    Purpose

    Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    T24N

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbE7a-RFP

    Plasmid

    #35313

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKJ0464

    Plasmid

    #228716

    Purpose

    Bacterial expression of T7 RNAP with E643G mutation; for protein production and purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Science 0, eadr6006

    Insert

    SUmo_T7pol_E643G
    

    Expression

    Bacterial

    Mutation

    E643G

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET24b-BR1.4

    Plasmid

    #199436

    Purpose

    expresses BAmRed1.4 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed1.4
    

    Tags

    his tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEVA221TadA*-T7RNAP

    Plasmid

    #167980

    Purpose

    Expresses fusion protein TadA*-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    TadA*-T7RNAP
    

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTuLys2CMR2

    Plasmid

    #53544

    Purpose

    Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance

    Depositor

    Lingchong You

    Article

    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

    Insert

    pLux
    

    Use

    Unspecified

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-SUMO-DogTag

    Plasmid

    #105629

    Purpose

    Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SUMO-DogTag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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