We narrowed to 10,636 results for: t7

1. pET11b-SpyCatcher-HA2

   Plasmid

   #165991

   Purpose

   SpyCatcher-fused influenza HA ministem with removable HisTag

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyCatcher-HA2
   

   Tags

   Foldon, HisTag, and SpyCatcher

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24b-BG1.3

   Plasmid

   #199437

   Purpose

   expresses BAmGreen1.3 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen1.3
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP450cam

   Plasmid

   #85085

   Purpose

   Expresses P450cam (CYP101A1) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450cam
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXTL-T7max-NanoLuc

   Plasmid

   #188313

   Purpose

   Expression of NanoLuc via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   NanoLuc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyLigase

   Plasmid

   #51722

   Purpose

   Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

   Depositor

   Mark Howarth

   Article

   Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

   Insert

   SpyLigase
   

   Tags

   His6 Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Beta-Lactamase

   Plasmid

   #62729

   Purpose

   Expresses Beta-Lactamase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Beta-Lactamase
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Deletion of periplasmic signal domain

   Promoter

   T7

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET_BGL3

   Plasmid

   #101912

   Purpose

   Expresses a β-glucosidase gene from Rhodothermus marinus in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7+LacO

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDM_T7_HisLacZomega

   Plasmid

   #118815

   Purpose

   T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   lacZ_
   

   Tags

   histag

   Expression

   Bacterial

   Available Since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Tags

   his tag and sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Ran-Q69L-mRFP1-polyA

    Plasmid

    #104560

    Purpose

    Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    Ran (Ran Mouse)
    

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Q69L

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET14b – lambda exonuclease

    Plasmid

    #104531

    Purpose

    bacterial expression of lambda exonuclease

    Depositor

    Charles Bell

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

    Insert

    λ exonuclease
    

    Tags

    His6 followed by a thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF-SeAvs3

    Plasmid

    #188805

    Purpose

    Codon optimized SeAvs3 with C-terminal His tag

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    SeAvs3
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-AcGFP-progerin

    Plasmid

    #86947

    Purpose

    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

    Depositor

    Alex Hewitt

    Insert

    AcGFP-progerin (LMNA Human)
    

    Use

    AAV

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    progerin sequence is LMNA gene with nucleotide C1…

    Promoter

    CMV and T7

    Available Since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Rx3(A7)

    Plasmid

    #35525

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    His6-Rx3(A7)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6his-WT gusA-pET28a+

    Plasmid

    #61156

    Purpose

    N -terminal His tagged wild-type GUS expression vector

    Depositor

    Andrew Ellington, Ichiro Matsumura

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    gusA
    

    Tags

    6x His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-HisYFP-Sp100

    Plasmid

    #53141

    Purpose

    E.coli expression of HisYFP tagged human Sp100 fragment

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    Sp100A fragment (SP100 Human)
    

    Tags

    His-YFP

    Expression

    Bacterial

    Mutation

    fragment (amino acids 71-480); deletion of L75

    Promoter

    T7

    Available Since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD/HisB (TIREVOL)-mNG

    Plasmid

    #189722

    Purpose

    titratable expression of mNeonGreen in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mNeonGreen
    

    Tags

    His-T7-EK

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST14-DoubleCatcher-H-Lock

    Plasmid

    #216285

    Purpose

    Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher H-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. luxB:Venus+luxA/pRSETB Duet

    Plasmid

    #197582

    Purpose

    BRET lux luciferase with Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBVenus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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