We narrowed to 10,636 results for: t7

1. 6xHis-MBP-huErCas12a

   Plasmid

   #174685

   Purpose

   Expresses 6xHis-MBP tagged ErCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huErCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV, NLS and NLS, 6xHis

   Expression

   Bacterial

   Mutation

   Q926R mutation is observed as indicated from the …

   Promoter

   T7

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFN18A-HaloTag-SpyCatcher

   Plasmid

   #206041

   Purpose

   HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Type

   Empty backbone

   Tags

   HaloTag and SpyCatcher-8His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SnoopTag-AffiHER2-SpyTag

   Plasmid

   #216280

   Purpose

   Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-AffiHER2-SpyTag
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ReNL_pRSETb

   Plasmid

   #89536

   Purpose

   Red color variant of bright luminescent protein for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Tags

   His X 6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Tags

   EGFP tag

   Expression

   Mammalian

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Plk1 FRET sensor c-jun substrate

   Plasmid

   #45203

   Depositor

   Michael Lampson

   Article

   Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

   Insert

   Plk1 FRET sensor with c-jun substrate
   

   Tags

   CyPET, YPET

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

   Plasmid

   #116954

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SETDB1

   Plasmid

   #25240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1, SETDB1_16 (SETDB1 Human)
   

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pBAD/HisB (TIREVOL)-sfGFP

   Plasmid

   #189675

   Purpose

   titratable expression of sfGFP in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   sfGFP
   

   Tags

   His-T7-EK

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcNB1

    Plasmid

    #89241

    Purpose

    Resurfaced Cell-penetrating GFP nanobody

    Depositor

    Brian McNaughton

    Article

    Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

    Insert

    pcNB1 (GFP)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPLP1H-mCf

    Plasmid

    #229984

    Purpose

    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPLP2H-mCf

    Plasmid

    #229985

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPLP3H-mCf

    Plasmid

    #229987

    Purpose

    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Tags

    his-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. wt HIV-1 integrase in pET15b

    Plasmid

    #61668

    Purpose

    wild type HIV-1 integrase in pET-15b

    Depositor

    Robert Craigie

    Article

    Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

    Insert

    wild type HIV-1 integrase
    

    Tags

    His

    Promoter

    T7 promotor

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. wg186Fluc

    Plasmid

    #177436

    Purpose

    In vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.

    Depositor

    Wendy Gilbert

    Article

    Rojas-Duran et al RNA. 2012 Dec;18(12):2299-305. doi: 10.1

    Insert

    Firefly luciferase
    

    Use

    Luciferase; For in vitro transcription

    Tags

    None

    Promoter

    T7

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK226

    Plasmid

    #71125

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-RILP

    Plasmid

    #74600

    Purpose

    used for expression in E. coli and purification

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab-interacting lysosomal protein (RILP Human)
    

    Tags

    FLAG, StrepII, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-6xHis-GFP(+12)

    Plasmid

    #199161

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+12)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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