We narrowed to 24,269 results for: fus
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Plasmid#119773PurposeConversion of C to T in potatoDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP1
Plasmid#183323PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP1 geneDepositorInsertTOP1
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
Tags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/Pd-mChe
Plasmid#183172PurposeConstruct for transient expression of plasmodesmata-targeted mCherry in plants.DepositorInsertPd-mCherry
TagsAtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR3
Plasmid#183291PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR3 geneDepositorInsertFGFR3
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) prME (JEV) sdm2
Plasmid#52881Purposeexpression of Japanese Encephalitis Virus VLPs in mammalian cellsDepositorAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1A
Plasmid#183337PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1A
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
TagsHisExpressionBacterialAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP GFP-VAMP7
Plasmid#45920DepositorAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1B
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralAvailable SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo DEST (w18-1)
Plasmid#17390Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, NeoDepositorTypeEmpty backboneUseGateway Cloning, Lentiviral, and RNAiAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP2A
Plasmid#183324PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP2A geneDepositorInsertTOP2A
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
TagsEGFP and HDELExpressionBacterial and PlantPromoter35SAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-V96 ELP
Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorInsertGFP fused to ELP (VPGVG) repeated 96 times
TagsGFP fused to N-terminal of ELPExpressionMammalianPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL1P5 ZmUbiP:GRF-GIF:NosT
Plasmid#198046PurposeMoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyPromoterZea mays (maize) ubiquitin promoterAvailable SinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLP4HS-mCf
Plasmid#229988PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.DepositorTypeEmpty backboneTagsHis6 and Twin-Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2H-mCf
Plasmid#229985PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP2S-mCf
Plasmid#229986PurposeLIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.DepositorTypeEmpty backboneTagsStrep-tag IIExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPLP3H-mCf
Plasmid#229987PurposeLIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.DepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B DBD INCENP GFP
Plasmid#45237PurposeTarget INCENP to centromeres via CENP-B DNA binding domain (DBD)DepositorInsertCenp-B-DBD, INCENP-Δcen, GFP
TagsDNA binding domain of CENP-B and GFPExpressionMammalianMutationCEN box deleted from INCENP (aa1-46)Available SinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Aurli_150841_GZG
Plasmid#162563PurposeInactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.DepositorInsertshble
UseUnspecifiedPromoterGAPDHAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK401 (Hygro-P2A-mAID-Nluc)
Plasmid#121196PurposeN-terminal taggingDepositorInsertHygro-P2A-mAID-Nluc
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-ALFA-tag-Gal4-VP16
Plasmid#199465PurposeExpression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.DepositorInsertGal4-ALFA-tag-VP16
ExpressionMammalianAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
TagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-N1-PHPLCd1
Plasmid#202679PurposePhotoactivatable lipid biosensorDepositorInsertPLCD1v2(1-170):PAmCherry1 (PLCD1 Human)
ExpressionMammalianAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_PARP1
Plasmid#183312PurposeAll-in-One CRISPRko system with a guide RNA that targets PARP1 geneDepositorInsertPARP1
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Expression scaffold for dCas9+sgRNA
Plasmid#171799PurposePICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNADepositorInsertPICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
UseCRISPRAvailable SinceAug. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSXND1-hPPT1
Plasmid#85817PurposeExpression of hPPT in mammalian cellsDepositorInserthuman PPT1 (PPT1 Human)
ExpressionMammalianAvailable SinceJan. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
A3A-Gam
Plasmid#119769PurposeConversion of C to T monocotyledons protoplastsDepositorInsertGam-APOBEC3A-nCas9-NLS-2XUGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DNMT3A
Plasmid#183283PurposeAll-in-One CRISPRko system with a guide RNA that targets DNMT3A geneDepositorInsertDNMT3A
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DNMT3B
Plasmid#183284PurposeAll-in-One CRISPRko system with a guide RNA that targets DNMT3B geneDepositorInsertDNMT3B
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
Plasmid#61966PurposePositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))DepositorInsertsN-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry
ExpressionBacterialPromoterPBAD and PLtetOAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-mMCL1-WT
Plasmid#45817DepositorInsertmMCL-1
ExpressionMammalianAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUB6-VSV-GS
Plasmid#190781PurposeExpresses VSV-GS in mammalian cellsDepositorInsertVSV-GS
TagsHAExpressionMammalianPromoterUbCAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)
Plasmid#55223DepositorTypeEmpty backboneTagsSNAPf and TwinStrepIIExpressionInsectAvailable SinceJuly 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-PBE
Plasmid#98161PurposeExpresses CRISPR base editor in protoplastsDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
ExpressionPlantPromoterUbiAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_IDH1
Plasmid#183300PurposeAll-in-One CRISPRko system with a guide RNA that targets IDH1 geneDepositorInsertIDH1
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G1
Plasmid#40174DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_IDH2
Plasmid#183301PurposeAll-in-One CRISPRko system with a guide RNA that targets IDH2 geneDepositorInsertIDH2
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only