We narrowed to 10,636 results for: t7

1. pDEST14-DoubleCatcher-ε-Lock

   Plasmid

   #216290

   Purpose

   Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ε-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMABC

   Plasmid

   #45398

   Depositor

   Katsu Murakami

   Article

   Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

   Inserts

   rpoA (rpoA Escherichia coli)
   rpoB (rpoB Escherichia coli)
   rpoC (rpoC Escherichia coli)
   

   Expression

   Bacterial

   Promoter

   None and T7

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-AqpZ-ALFA

   Plasmid

   #212310

   Purpose

   Expression of AqpZ with ALFA tag

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   Aquaporin Z
   

   Tags

   ALFA tag and Strep-Tag II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET_BGL1

   Plasmid

   #101910

   Purpose

   Expresses a β-glucosidase gene from Thermomicrobium roseum in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7+LacO

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30b-7Y-SrtA

   Plasmid

   #105601

   Purpose

   expresses 7Y sortase A variant in E.coli

   Depositor

   Stephanie Dougan

   Article

   Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

   Insert

   S.aureus Sortase A, 7Y mutant
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pGEM-SNAP-TRIM21

   Plasmid

   #196181

   Purpose

   For in vitro transcription of SNAP-TRIM21 mRNA

   Depositor

   Binyam Mogessie

   Article

   Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

   Insert

   SNAP-TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Neq2X

   Plasmid

   #182365

   Purpose

   Expresses Neq2X DNA polymerase

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

   Insert

   Neq2X
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbB7k-GFP

   Plasmid

   #35360

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEVA221AID-T7RNAP

    Plasmid

    #167977

    Purpose

    Expresses fusion protein AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    AID-T7RNAP
    

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet-1∆HisTag_Nef_MCS2

    Plasmid

    #66079

    Purpose

    Single vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli system

    Depositor

    Dieter Willbold

    Article

    Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

    Insert

    Nef
    

    Tags

    6xHis and HRV 3C Site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET28-his6-HA3-SNAPf

    Plasmid

    #188941

    Purpose

    T7 expression vector for making his6- and HA3-tagged SNAP protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-SNAPf
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. EcMBP165-cpGFP.PPYF.pRSET

    Plasmid

    #33372

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    MBP based Maltose Biosensor
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2BK120C

    Plasmid

    #75332

    Purpose

    Bacteria expression of H2BK120C

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    H2BK120C (h2bc11.S Frog)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated; K120C

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDL1

    Plasmid

    #182263

    Purpose

    Gibson cloning vector for synthesis of single and double stranded RNA

    Depositor

    Daniel Lobo

    Article

    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

    Type

    Empty backbone

    Use

    Gibson cloning vector for synthesis of single and…

    Promoter

    T3 and SP6 for sense and antisense riboprobe synt…

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AgAK

    Plasmid

    #71290

    Purpose

    Expression of A. gambiae adenosine kinase

    Depositor

    Vern Schramm

    Article

    Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

    Insert

    Adenosine Kinase
    

    Tags

    6X Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET19b+H2B

    Plasmid

    #157809

    Purpose

    Expression of human histone H2B

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H2B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pfMDH_WT

    Plasmid

    #67721

    Purpose

    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

20. OeNL_pRSETb

    Plasmid

    #89529

    Purpose

    Orange color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Tags

    His X 6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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