We narrowed to 10,636 results for: t7

1. pBbS7c-RFP

   Plasmid

   #35320

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET_BGL2

   Plasmid

   #101911

   Purpose

   Expresses a β-glucosidase gene from Thermobaculum terrenum in E. coli under the control of the pET system.

   Depositor

   Steve Shih

   Article

   Husser et al ACS Synth Biol. 2018 Mar 16;7(3):933-944

   Insert

   β-glucosidase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7+LacO

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIA1400

   Plasmid

   #172519

   Purpose

   Expression of SARS-COV-2 Nsp12 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24b-BR1.0

   Plasmid

   #199435

   Purpose

   expresses BAmRed1.0 in bacterial cells

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMRed
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22-HT/spoR

   Plasmid

   #73631

   Purpose

   Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

   Depositor

   T. J. Kappock, James Williamson

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   guanylate kinase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINK-UBE2S

   Plasmid

   #66714

   Purpose

   Expresses UBE2S (1-196) in E. coli

   Depositor

   David Komander

   Article

   Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

   Insert

   e2-epf (UBE2S )
   

   Tags

   GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBbE7c-RFP

   Plasmid

   #35314

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQA-LP574

   Plasmid

   #222953

   Purpose

   Expresses R120A LoaP

   Depositor

   Adrian Ferré-D'Amaré

   Article

   Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

   Insert

   antiterminator protein LoaP
   

   Tags

   10xHIS-SUMO

   Expression

   Bacterial

   Mutation

   mutated Arginine 120 to Alanine

   Promoter

   T7

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SdrG_N2N3-HIS-ybbr

   Plasmid

   #101238

   Purpose

   SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   SdrG_N2N3
   

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-clbH-A1

    Plasmid

    #114149

    Purpose

    Expresses ClbH-A1

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbH-A1
    

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Mutation

    Contains only the A1 domain of ClbH

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNRB150

    Plasmid

    #36451

    Depositor

    Douglas Bishop

    Article

    Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

    Insert

    DMC1 (DMC1 Budding Yeast)
    

    Tags

    6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Tras light chain

    Plasmid

    #216294

    Purpose

    Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Pert Light chain

    Plasmid

    #216310

    Purpose

    Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Pert Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCSG7-dNmeCas9

    Plasmid

    #71475

    Purpose

    bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-Inactive-NmeCas9
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A H588A

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH6-spTorA-mCherry

    Plasmid

    #169040

    Purpose

    mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-mCherry
    

    Tags

    6xHis and spTorA (signal peptide of E. coli TorA)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCellFree_G03 CYP2A13

    Plasmid

    #67103

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2A13 (CYP2A13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pRset-OppA

    Plasmid

    #207869

    Purpose

    Bacterial expression of poly-his-tagged L. lactic OppA

    Depositor

    Mathew Tantama

    Article

    Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

    Insert

    OppA
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p_SNIPR_target_WT

    Plasmid

    #139465

    Purpose

    T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_WT
    

    Expression

    Bacterial

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-his6-HA3-HALO

    Plasmid

    #188943

    Purpose

    T7 expression vector for making his6- and HA3-tagged HALO protein

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    his6-HA3-HALO
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4092694

    Plasmid

    #153907

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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