We narrowed to 10,636 results for: t7

1. pCellFree_G03 RAD54B

   Plasmid

   #67124

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RAD54B (RAD54B Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET_scFv_QLuc

   Plasmid

   #183261

   Purpose

   Express scFv_QLuc in bacteria cells

   Depositor

   Huiwang Ai

   Article

   Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

   Insert

   scFv_QLuc
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL2472

   Plasmid

   #36853

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   TP53-TALEN_Right (Tp53 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. TAL2348

   Plasmid

   #36768

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   TP53-TALEN_Left (Tp53 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pAAH5

   Plasmid

   #41829

   Purpose

   Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Expression

   Bacterial and Yeast

   Promoter

   T7,TEF

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGC479

   Plasmid

   #42601

   Depositor

   Jane Hubbard

   Article

   Korta et al Development. 2012 Mar;139(5):859-70. doi

   Insert

   daf-15 (daf-15 Nematode)
   

   Use

   RNAi

   Promoter

   T7

   Available Since

   April 19, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET-51b(+) ChemoG1

   Plasmid

   #193799

   Purpose

   Protein production of ChemoG1 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG1
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSETA_crE6G2

   Plasmid

   #172929

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6G2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Val317Gly, Met316Gly

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NTERY00

   Plasmid

   #184109

   Purpose

   Nanopore-addressable protein reporter for expression in bacterial cells.

   Depositor

   Jeff Nivala

   Article

   Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

   Insert

   NTERY00
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NTERY02

    Plasmid

    #184120

    Purpose

    Nanopore-addressable protein reporter for expression in bacterial cells.

    Depositor

    Jeff Nivala

    Article

    Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

    Insert

    NTERY02
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

    Plasmid

    #116965

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD/HisB (TIREVOL)-mEos3.2

    Plasmid

    #189720

    Purpose

    titratable expression of mEos3.2 in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    mEos3.2
    

    Tags

    His-T7-EK

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-Vx3(A7)

    Plasmid

    #35526

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    Vx3(A7)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTYB2-PAB1

    Plasmid

    #37234

    Depositor

    Jon Lorsch

    Article

    Park et al EMBO J. 2011 Jan 19;30(2):302-16. Epub 2

    Insert

    PAB1 (PAB1 Budding Yeast)
    

    Tags

    intein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pJK438

    Plasmid

    #71590

    Purpose

    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    citrate synthase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-hDAGLb-V5

    Plasmid

    #87675

    Purpose

    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

    Depositor

    Pat Doherty

    Insert

    Human Diacylglycerol Lipase Beta (DAGLB Human)
    

    Tags

    HA and V5 tag

    Expression

    Mammalian

    Mutation

    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

    Promoter

    T7

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFS_0143-pET30-Fusicatenibacter_saccharivorans

    Plasmid

    #116952

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Expression

    Insect

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbA7a-RFP

    Plasmid

    #35316

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

    Plasmid

    #116964

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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