Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 24,269 results for: fus

Showing: 8201 - 8240 of 24269 results

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CROP_C9_Puro_RET

Plasmid

#183319

Purpose

All-in-One CRISPRko system with a guide RNA that targets RET gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

RET

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_RARA

Plasmid

#183318

Purpose

All-in-One CRISPRko system with a guide RNA that targets RARA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

RARA

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PSMB5

Plasmid

#183317

Purpose

All-in-One CRISPRko system with a guide RNA that targets PSMB5 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PSMB5

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PIK3CD

Plasmid

#183316

Purpose

All-in-One CRISPRko system with a guide RNA that targets PIK3CD gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PIK3CD

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_PIGF

Plasmid

#183315

Purpose

All-in-One CRISPRko system with a guide RNA that targets PIGF gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

PIGF

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MET

Plasmid

#183310

Purpose

All-in-One CRISPRko system with a guide RNA that targets MET gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MET

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MAP2K2

Plasmid

#183309

Purpose

All-in-One CRISPRko system with a guide RNA that targets MAP2K2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MAP2K2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_MAP2K1

Plasmid

#183308

Purpose

All-in-One CRISPRko system with a guide RNA that targets MAP2K1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

MAP2K1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_LCK

Plasmid

#183307

Purpose

All-in-One CRISPRko system with a guide RNA that targets LCK gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

LCK

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_KIT

Plasmid

#183306

Purpose

All-in-One CRISPRko system with a guide RNA that targets KIT gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

KIT

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_KDR

Plasmid

#183305

Purpose

All-in-One CRISPRko system with a guide RNA that targets KDR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

KDR

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_JAK3

Plasmid

#183304

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK3

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_JAK1

Plasmid

#183302

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FYN

Plasmid

#183295

Purpose

All-in-One CRISPRko system with a guide RNA that targets FYN gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FYN

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CYP17A1

Plasmid

#183279

Purpose

All-in-One CRISPRko system with a guide RNA that targets CYP17A1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CYP17A1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CSF3R

Plasmid

#183278

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF3R gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF3R

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CDK6

Plasmid

#183276

Purpose

All-in-One CRISPRko system with a guide RNA that targets CDK6 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CDK6

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CDK4

Plasmid

#183275

Purpose

All-in-One CRISPRko system with a guide RNA that targets CDK4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CDK4

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BTK

Plasmid

#183274

Purpose

All-in-One CRISPRko system with a guide RNA that targets BTK gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BTK

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_BCL2

Plasmid

#183272

Purpose

All-in-One CRISPRko system with a guide RNA that targets BCL2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BCL2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_AR

Plasmid

#183271

Purpose

All-in-One CRISPRko system with a guide RNA that targets AR gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AR

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_ALK

Plasmid

#183270

Purpose

All-in-One CRISPRko system with a guide RNA that targets ALK gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ALK

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CROP_C9_Puro_ADA

Plasmid

#183269

Purpose

All-in-One CRISPRko system with a guide RNA that targets ADA gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ADA

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pb mD3S4-dN

Plasmid

#180252

Purpose

P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

Depositor

Timothy Springer

Article

Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

Insert

D3 of P. berghei HAP2 with N-glycosylation sites mutated

Tags

6His

Expression

Mammalian

Mutation

N516T, S533N and N539Q

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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4E1H.95_LUMABS

Plasmid

#155208

Purpose

Expresses 4E1H.95_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3H1L_02.395

Plasmid

#155198

Purpose

Expresses 3H1L_02.395 in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3H1L_02.395

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLz33_CsoS2_NTD_GFP

Plasmid

#140861

Purpose

His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD fused to GFP

Tags

Hexahistidine tag and Superfolder GFP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p415:iCas9

Plasmid

#138266

Purpose

Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mTN3-GGS6-dCas9

Use

CRISPR

Expression

Yeast

Mutation

Cas9-D10A, H840A

Promoter

GalL

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(NT)

Plasmid

#138258

Purpose

Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-hsg(NT:NT)

Plasmid

#138260

Purpose

Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-FeGFP

Plasmid

#138261

Purpose

Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

eGFP

Use

CRISPR

Expression

Mammalian

Promoter

None

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-ysg(NT)

Plasmid

#138263

Purpose

Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

Nontargeting sgRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T+1X

Plasmid

#108188

Purpose

PCNA1-PdX fusion protein

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FH95pUG+p97W (B8)

Plasmid

#74017

Purpose

lentiviral expression of Psd95 shRNA, and EGFP-SAP97beta fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAF-MutL(R95F)

Plasmid

#86608

Purpose

E. coli Mutl(R95F) expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL(R95F)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

R95F

Promoter

T7

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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