We narrowed to 10,636 results for: t7

1. TAL2369

   Plasmid

   #36780

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   NCOR1-TALEN_Right (Ncor1 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pBAD/HisB (TIRSTD)-mNG

   Plasmid

   #189721

   Purpose

   titratable expression of mNeonGreen in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   mNeonGreen
   

   Tags

   His-T7-EK

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-nGFPMNase

   Plasmid

   #187826

   Purpose

   express nGFPMNase protein in E coli

   Depositor

   Yikang Rong

   Article

   Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

   Insert

   nGFPMNASE
   

   Tags

   his tag

   Expression

   Bacterial

   Promoter

   T7 Promotor

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. TAL2220

   Plasmid

   #36679

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   RECQL4-TALEN_Left (Recql4 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pCellFree_G03 SP2

   Plasmid

   #67109

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SP2 (SP2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pAAH6

   Plasmid

   #41830

   Purpose

   Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Expression

   Bacterial and Yeast

   Promoter

   T7,TEF

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSHS293

   Plasmid

   #87348

   Purpose

   D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/D435C/C574S/E945C

   Promoter

   T7

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbA7c-RFP

   Plasmid

   #35317

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 USF2

   Plasmid

   #67069

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   USF2 (USF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

    Plasmid

    #116953

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-Histone H1

    Plasmid

    #45083

    Depositor

    Phillip Newmark

    Article

    Rouhana et al Development. 2014 Jul;141(13):2592-601.

    Insert

    Histone H1
    

    Use

    RNAi; In vitro transcription

    Promoter

    T7 sense, T3 antisense

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCellFree_G03 VPS29

    Plasmid

    #67052

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    VPS29 (VPS29 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pMCSG7(gfcB)

    Plasmid

    #176196

    Purpose

    gfcB protein expression

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcB
    

    Tags

    6xHis-TeV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p_SNIPR_target_GUmut33AtoG

    Plasmid

    #139468

    Purpose

    T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    Target_mutGU33
    

    Expression

    Bacterial

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MS2_SC2_S_Omicron

    Plasmid

    #180229

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Omicron (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET21-tVYN-Nt∆

    Plasmid

    #154431

    Purpose

    Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-NtYcgRΔ-NLucΔ4
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbA7k-RFP

    Plasmid

    #35318

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TAL2456

    Plasmid

    #36845

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    VHL-TALEN_Left (Vhl Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pSC-hTDG-PreE2

    Plasmid

    #52272

    Purpose

    Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Tags

    Ubc9-GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSHS248

    Plasmid

    #87354

    Purpose

    S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S/S867C/N1054C

    Promoter

    T7

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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