We narrowed to 9,193 results for: Pol;

1. psicheck-2-Casp8-mod

   Plasmid

   #48171

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> ATT) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of CASP8 (CASP8 Human)
   

   Use

   Luciferase

   Mutation

   G/T MUTATION IN ATG OF UORF

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

2. psicheck-2-Mc2r-mod

   Plasmid

   #48173

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of MC2R (MC2R Human)
   

   Use

   Luciferase

   Mutation

   A/G MUTATION IN ATG OF UORF

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

3. psicheck-2-Kdr-mod

   Plasmid

   #48177

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of 2KDR (MC2R Human)
   

   Use

   Luciferase

   Mutation

   A/G MUTATION IN ATG OF UORF

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

4. psicheck-2-Hsdl2-mod

   Plasmid

   #48180

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of HSDL2 (Hsdl2 Mouse)
   

   Use

   Luciferase

   Mutation

   A/T MUTATION IN ATG OF UORF

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. psicheck-2-Hsdl2

   Plasmid

   #48179

   Purpose

   contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   5" UTR of HSDL2 (Hsdl2 Mouse)
   

   Use

   Luciferase

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAAV_GR-RE_MLPmin_BC1094-luc2

   Plasmid

   #227114

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK5785

   Plasmid

   #219753

   Purpose

   pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pGAP - nnLuz_v4 - tAOX
   

   Use

   Luciferase

   Expression

   Yeast

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

   Plasmid

   #111568

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Has Service

   AAV5

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNK5664

   Plasmid

   #219747

   Purpose

   DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pCMV - nnLuz_v4 - tSV40
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB80-hsKIF5B(1-560)Rigor-L-2xHA

    Plasmid

    #193718

    Purpose

    Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB80-hsKIF5B(1-353)GCN4-L-GFP

    Plasmid

    #193714

    Purpose

    Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Human, Synthetic)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-353);

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB80-hsKIF5B(1-353)-GCN4-L-mCherry

    Plasmid

    #193715

    Purpose

    Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-353); mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid

    #140893

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid

    #107581

    Purpose

    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

    Depositor

    Paul Freemont

    Article

    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pdh promoter Bacillus megaterium DSM319

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-BFP-Mito

    Plasmid

    #237398

    Purpose

    Lentivirus plasmid for the stable expression of mitochondrial matrix-localized BFP in mammalian cells

    Depositor

    James Hurley

    Article

    Rose et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

    Insert

    COX8A (COX8A Human)
    

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Aug. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-GFP-IKBalpha-wt

    Plasmid

    #15263

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEv-Fat4-citrine

    Plasmid

    #112854

    Purpose

    Constitutive expression of human fat4 protein fused to citrine fluorescent protein

    Depositor

    David Sprinzak

    Article

    Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

    Insert

    FAT4 (FAT4 Human)
    

    Tags

    Citrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TDP-43_NTD1-80_E17R

    Plasmid

    #104479

    Purpose

    bacterial expression of TDP-43 NTD E17R

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17R

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSEP-LAMP1-RFP

    Plasmid

    #167970

    Purpose

    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

    Depositor

    Juan Bonifacino

    Article

    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    SEP and mRFP

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV_AR-RE_MLPmin_BC1011-luc2

    Plasmid

    #227106

    Purpose

    Androgen receptor response element for luciferase and barcode assays; barcode BC1011

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-Dlx-SynaptoTAG

    Plasmid

    #210729

    Purpose

    AAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.

    Depositor

    Wei Xu

    Article

    Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

    Insert

    EGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Rat, Synthetic)
    

    Use

    AAV

    Promoter

    Dlx

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

    Plasmid

    #174515

    Purpose

    Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    Mm PylS
    MmtRNA-Pyl-opt(UGA)
    

    Expression

    Bacterial

    Mutation

    ASL from the serT gene (UGA anticodon) and recoded

    Promoter

    GlnS and lpp

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLJM1 TMEM192-Flag-FKBP

    Plasmid

    #112743

    Purpose

    express TMEM192-Flag-FKBP

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    TMEM192-Flag-FKBP (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag, FKBP

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SaCas9 GFP V3

    Plasmid

    #226962

    Purpose

    CBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge