We narrowed to 10,636 results for: t7

1. pNIC28-BpGmhA

   Plasmid

   #125589

   Purpose

   Overexpression plasmid for production of GmhA from Burkholderia pseudomallei

   Depositor

   Nicholas Harmer

   Article

   Harmer et al J Mol Biol. 2010 Jul 16;400(3):379-92. d

   Insert

   BpGmhA (gmhA Burkholderia pseudomallei)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTXTL-T7max-LuxB

   Plasmid

   #188306

   Purpose

   Expression of LuxB via a T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   luxB
   

   Use

   Luciferase and Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBAD/HisB (TIREVOL)-PAmCherry1

   Plasmid

   #189718

   Purpose

   titratable expression of PAmCherry1 in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   PAmCherry1
   

   Tags

   His-T7-EK

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTXTL-T7max-HisGFPHiBiT

   Plasmid

   #188316

   Purpose

   Expression of GFP-HiBiT via T7 promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

   Insert

   GFP-HiBiT fusion
   

   Use

   Luciferase and Synthetic Biology

   Tags

   8x His-tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIA1402

   Plasmid

   #172403

   Purpose

   Expression of SARS-COV-2 Nsp12 Y129A in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Y129A

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFLIP38

   Plasmid

   #65616

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. msEGFP-PACR_pRSETB

   Plasmid

   #55775

   Purpose

   Genetically encoded caged Ca2+

   Depositor

   Takeharu Nagai

   Article

   Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

   Insert

   PACR
   

   Tags

   6xHis and msEGFP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. PCx35IL-pET42

   Plasmid

   #42916

   Depositor

   John O'Brien

   Article

   Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

   Insert

   Connexin 35 cytoplasmic loop
   

   Tags

   6x His, GST, and S-tag

   Expression

   Bacterial

   Mutation

   cytoplasmic loop is aa 101-179

   Promoter

   T7

   Available Since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TAL2284

   Plasmid

   #36719

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   NCOR2-TALEN_Left (Ncor2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

    Plasmid

    #116966

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TAL2409

    Plasmid

    #36812

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NBN-TALEN_Right (Nbn Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pBS-SLBP

    Plasmid

    #45082

    Depositor

    Phillip Newmark

    Article

    Rouhana et al Development. 2014 Jul;141(13):2592-601.

    Insert

    Stem Loop Binding Protein
    

    Use

    RNAi; In vitro transcription

    Promoter

    T7 sense, T3 antisense

    Available Since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only

13. TAL2454

    Plasmid

    #36843

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    SIRT6-TALEN_Left (Sirt6 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. TAL2455

    Plasmid

    #36844

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    SIRT6-TALEN_Right (Sirt6 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET15b-eIF4E

    Plasmid

    #37228

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    eIF4E
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Tags

    8xhistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIA1408

    Plasmid

    #172405

    Purpose

    Expression of SARS-COV-2 Nsp12 D760A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D760A

    Promoter

    T7

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAH2

    Plasmid

    #41826

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Expression

    Bacterial and Yeast

    Promoter

    T7, TEF

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TAL2368

    Plasmid

    #36779

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Left (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only