We narrowed to 10,636 results for: t7

1. pCellFree_G03 SREBF2

   Plasmid

   #67129

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SREBF2 (SREBF2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET21a(+)-HpDsbG

   Plasmid

   #39329

   Depositor

   SeWon Suh

   Article

   Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

   Insert

   oxidoreductase
   

   Tags

   His-Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

3. HDGF2

   Plasmid

   #32021

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HDGF2 (HDGFL2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   PWWP domain only (amino acid residues 1-93)

   Promoter

   T7

   Available Since

   Jan. 3, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pCDF-CcAvs4

   Plasmid

   #188808

   Purpose

   Codon optimized CcAvs4 with C-terminal His tag

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   KpAvs4
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDF-EcAvs4

   Plasmid

   #188806

   Purpose

   Codon optimized EcAvs4 with C-terminal His tag

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EcAvs4
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMPO 61

   Plasmid

   #69869

   Purpose

   Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, MCS M13 replication origin

   Depositor

   Eduardo Santero

   Article

   Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMPO 58

   Plasmid

   #68362

   Purpose

   Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, ColE1 replication origin

   Depositor

   Eduardo Santero

   Article

   Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCellFree_G03 SP6

   Plasmid

   #67074

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SP6 (SP6 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. 5KCH (SETDB1)

   Plasmid

   #101881

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1 (SETDB1 Human)
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pVY-3 sfGFP-tag3

    Plasmid

    #194618

    Purpose

    fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    sfGFP-K(GGSKKRKKR)3
    

    Tags

    KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TAL2218

    Plasmid

    #36677

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MUTYH-TALEN_Left (Mutyh Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pET28b-MseLig1

    Plasmid

    #107071

    Purpose

    Expresses M. sedula DNA Ligase 1 in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    DNA ligase 1
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NTERY06

    Plasmid

    #184124

    Purpose

    Nanopore-addressable protein reporter for expression in bacterial cells.

    Depositor

    Jeff Nivala

    Article

    Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

    Insert

    NTERY06
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. UbG75C

    Plasmid

    #37194

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    Ubiquitin G75C mutant (RBX1 Human)
    

    Tags

    No tag

    Expression

    Bacterial

    Mutation

    Glycine 75 changed to Cystine

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pTXG-hTDG

    Plasmid

    #52281

    Purpose

    Bacterial expression of human TDG C-terminally fused to GyrA intein and GST

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    hTDG (TDG Human)
    

    Tags

    GyrA-intein-GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TAL2285

    Plasmid

    #36720

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR2-TALEN_Right (Ncor2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pAW206

    Plasmid

    #113246

    Purpose

    E. coli expression vector for IHFa K15A + IHFb

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    IHFa K15A (ihfA E. coli)
    IHFb
    

    Expression

    Bacterial

    Mutation

    K15A

    Promoter

    T7

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EcYajL pET15b

    Plasmid

    #60716

    Purpose

    Recombinant protein expression of YajL

    Depositor

    Mark Wilson

    Article

    Wilson et al J Mol Biol. 2005 Oct 28;353(3):678-91. E

    Insert

    YajL
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

20. Hox A13a

    Plasmid

    #36463

    Depositor

    Robert Ho

    Article

    Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

    Insert

    hoxA13a (hoxa13a Zebrafish)
    

    Use

    Riboprobe synthesis

    Mutation

    contains aa102-290; M107T

    Promoter

    T7

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only