We narrowed to 9,193 results for: Pol;

1. CIB-VP64 (B1016)

   Plasmid

   #92037

   Purpose

   Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1-VP64 (CIB1 Mustard Weed)
   

   Tags

   Fusion of plant CIB1 with VP64 activation domain.…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaV1.3e[8a,11,31b,Δ32,Δ42a]

   Plasmid

   #49332

   Purpose

   Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

   Depositor

   Diane Lipscombe

   Article

   Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

   Insert

   CACNA1D (Cacna1d Rat)
   

   Expression

   Mammalian

   Mutation

   Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

   Promoter

   CMV

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AT-U1

   Plasmid

   #112059

   Purpose

   U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   U1 (RNU1-1 Human)
   

   Expression

   Mammalian

   Mutation

   1 copy of Riboglow tag (truncated variant A) near…

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-Puro-IKBalpha-wt

   Plasmid

   #15290

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pHRIG-AktDN

   Plasmid

   #53597

   Purpose

   lentiviral expression of dominant negative mouse Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   Dominant negative (K179M)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

   Plasmid

   #164471

   Purpose

   Expresses sgRNA targeting human GJA1 locus

   Depositor

   Markus Kuehn

   Article

   Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

   Insert

   Gap junction alpha-1 (GJA1) (GJA1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_AR-RE_MLPmin_BC1014-luc2

   Plasmid

   #227108

   Purpose

   Androgen receptor response element for luciferase and barcode assays; barcode BC1014

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_ER-RE_MLPmin_BC1063-luc2

   Plasmid

   #227109

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

   Plasmid

   #149714

   Purpose

   Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   BHLHE40 (BHLHE40 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBetaActin-FKBP-mScarlet-Dync1h1motor

    Plasmid

    #191330

    Purpose

    Expresses the fluorescently labeled dynein motor domain fused to dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-Dync1h1motor (Dync1h1 Mouse)
    

    Expression

    Mammalian

    Mutation

    DYNC1H1 motor domain aa 1453-4644

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNK5712

    Plasmid

    #219752

    Purpose

    pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - nnH3H_v2 - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Zeo_BACE1

    Plasmid

    #227105

    Purpose

    BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    BACE1 (BACE1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

    Plasmid

    #122957

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Promoter

    CAG promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mScarlet-hCenpC

    Plasmid

    #171490

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    CenpC (CENPC Human)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-Neo-TetO-Cdk12_Untagged

    Plasmid

    #127178

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Promoter

    TetO Promoter

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-Flag-Rheb-4Rs

    Plasmid

    #165022

    Purpose

    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb-4Rs (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    change lysine residues on position 109, 135, 151,…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR8-PHF19

    Plasmid

    #68864

    Purpose

    GW entry vector for PHF19

    Depositor

    Adrian Bracken

    Article

    Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Gateway entry vector

    Promoter

    none

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pBK_Gal4-DBD-GR(NR3C1)

    Plasmid

    #227100

    Purpose

    GR receptor overexpression for luciferase and barcode assays

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    GR (NR3C1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNK5867

    Plasmid

    #219751

    Purpose

    pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - mcitHispS - tAOX
    

    Expression

    Yeast

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNK1508

    Plasmid

    #219750

    Purpose

    pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - npgA - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLV-mTurquoise-MLC-IRES-neo

    Plasmid

    #85145

    Purpose

    lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mTurquoise

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. TRE-Lepr_Short-IRES-eGFP

    Plasmid

    #196935

    Purpose

    Doxycycline inducible expression of short Lepr (no signaling) with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    short isoform, lack of signaling casacade

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET28_6xHis-PARP1delCAT

    Plasmid

    #173942

    Purpose

    Bacterial expression of PARP1 lacking CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delCAT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 661-1014

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. W118-1_Flag-hIRAK1

    Plasmid

    #180405

    Purpose

    lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    IRAK1 (IRAK1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pX020

    Plasmid

    #167146

    Purpose

    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal caffeoylpiruvate hydrolase, nnCPH
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV_ER-RE_MLPmin_BC1065-luc2

    Plasmid

    #227111

    Purpose

    Estrogen receptor response element for luciferase and barcode assays; barcode BC1065

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered ER response element linked to MLP minimal promoter driving barcode 1065 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28_6xHis-PARP1delWGR

    Plasmid

    #173940

    Purpose

    Bacterial expression of PARP1 lacking WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid

    #172473

    Purpose

    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    

    Use

    Piggybac transposon

    Tags

    polybasic and CAAX motif from Kras, tdKatushka2, …

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. PARP1-c009

    Plasmid

    #216872

    Purpose

    Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

    Depositor

    Lari Lehtiö

    Article

    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

    Insert

    PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
    

    Expression

    Bacterial

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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