We narrowed to 9,193 results for: Pol;

1. hRAD18 dC2-EGFP

   Plasmid

   #68826

   Purpose

   Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

   Depositor

   Satoshi Tateishi

   Article

   Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

   Insert

   human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

   Plasmid

   #122954

   Purpose

   Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NLB (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Promoter

   CAG promoter

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

   Plasmid

   #63139

   Purpose

   Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

   Depositor

   Titia Sixma

   Article

   Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

   Inserts

   Ring1b (Ring1 Mouse)
   Bmi1 (Bmi1 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-109 and Residues 1-159

   Promoter

   tac

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pGM79

   Plasmid

   #220171

   Purpose

   CRISRPi guide RNA against MARCHF5

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   MARCHF5 (sgRNA) (MARCHF5 Human)
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKIV-DCTK75E

   Plasmid

   #126423

   Purpose

   A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed lysine 75 to glutamic acid

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1delZn3

   Plasmid

   #173936

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3 (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK3203

   Plasmid

   #219749

   Purpose

   DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   pCMV - mcitHispS - tSV40
   

   Expression

   Mammalian

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET30a-His6-ABP-LOXCATmut

    Plasmid

    #139972

    Purpose

    A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2281

    Plasmid

    #111902

    Purpose

    ssAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple346 (NEFM Human)
    

    Use

    AAV

    Promoter

    Ple346 (NEFM MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Flag MFF iso2 S172A

    Plasmid

    #74392

    Purpose

    Flag tagged human S172A MFF isoform 2 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Frt-hspB1

    Plasmid

    #63092

    Purpose

    Expression of HSP1 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid

    #221081

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET32a-HD39Q

    Plasmid

    #11514

    Depositor

    Pamela Bjorkman

    Article

    Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

    Insert

    huntingtin, exon 1, 39 glutamines (HTT Human)
    

    Tags

    His, His (out of frame), and Trx

    Expression

    Bacterial

    Mutation

    huntingtin exon 1 with 39 glutamines, vector m…

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid

    #69842

    Purpose

    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    K41M-S285A-T289A

    Promoter

    CMV

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse a5 full length

    Plasmid

    #217810

    Purpose

    Full length mouse integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (Itga5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28_6xHis-PARP1delZn1

    Plasmid

    #173933

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_6xHis-PARP1-HD

    Plasmid

    #174809

    Purpose

    Bacterial expression of isolated PARP1 HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-HD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPICZ-mouseGIRK2-eGFP-StII-6xHis

    Plasmid

    #124277

    Purpose

    Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGEX-6P-1_WT RAD23A

    Plasmid

    #201456

    Purpose

    Expresses human RAD23A with an N-terminal GST tag in E. coli

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    tac promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Frt-hspB8

    Plasmid

    #63099

    Purpose

    Expression of HSPB8 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid

    #138525

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    Control non-PRC2 binding A-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pBactin-Nedd1-mNeonGreen

    Plasmid

    #196864

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mNeonGreen (Nedd1 Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRS-mTagRFP-TUBA1B

    Plasmid

    #62850

    Purpose

    Donor vector for gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    mTagRFP-TUBA1B (TUBA1B Synthetic, Human)
    

    Use

    Yeast-e.coli shuttle vector

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJ4M/TDP-43 S48E

    Plasmid

    #104481

    Purpose

    bacterial expression of TDP-43 S48E

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    S48E

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pBacMam2-DiEx-LIC-C entry vector

    Plasmid

    #111752

    Purpose

    Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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