We narrowed to 9,193 results for: Pol;

1. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

   Plasmid

   #91807

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   PreScission Protease site (LEVLFQGP) inserted bet…

   Promoter

   Endogenous Rpb1 promoter

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

   Plasmid

   #91818

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1355 and lysine 1435 to alanines; …

   Promoter

   T7

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJM1-Flag-Rheb-12Rs

   Plasmid

   #165023

   Purpose

   expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb-12Rs (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   All the lysine residues except K19 and K120 are c…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1A Prk-

   Plasmid

   #162694

   Purpose

   p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Prk inactive (K20M S21A native protein, this map …

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_gRNA_H19-DMR-2

   Plasmid

   #159930

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6, CBh

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Mm_Plk5(300-595) PBD domain

   Plasmid

   #136316

   Purpose

   Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (Plk5 Mouse)
   

   Expression

   Mammalian

   Mutation

   fragment 300-595

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmirGLO-3'UTR-IL22

   Plasmid

   #153070

   Purpose

   Human IL-22 3'UTR region cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   3' UTR region of IL-22 gene (IL22 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDF11-SFPQ(214-598)_quadruple_mutant

   Plasmid

   #135441

   Purpose

   Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

   Depositor

   Charles Bond

   Article

   Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

   Insert

   splicing factor proline and glutamine rich (SFPQ Human)
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   L535A, L539A, L546A, M549A

   Promoter

   T7

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBacMam2-DiEx-LIC-N entry vector

    Plasmid

    #111751

    Purpose

    Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCRII apoBa

    Plasmid

    #121897

    Purpose

    Plasmid for making zebrafish apoBa in situ probe

    Depositor

    Steven Farber

    Article

    Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

    Insert

    ApoBa (apoba Zebrafish)
    

    Use

    Unspecified

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1 Dynamin1 T78R L84R T92R V118R

    Plasmid

    #112106

    Purpose

    Mammalian expression plasmid of GFP-tagged Dynamin protein.

    Depositor

    Justin Taraska

    Article

    Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

    Insert

    Dynamin1 (DNM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T78R L84R T92R V118R

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. hASXL(1-115)-luciferase

    Plasmid

    #106427

    Purpose

    Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    addition sex combs like 1 ASXL1 (ASXL1 Human)
    

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    amino acids 1-115 fused to luciferase

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mOC-STAMP (N162D)_3xFlag-Myc-CMV26

    Plasmid

    #73578

    Purpose

    Express mouse OC-STAMP ((N162D) in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Insert

    OC-STAMP (Ocstamp Mouse)
    

    Tags

    3x Flag and c-Myc tag

    Expression

    Mammalian

    Mutation

    changed asparagin (N) 162 to Aspartic Acid (D)

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-GST-CHD5-WT-FRAG

    Plasmid

    #68873

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    331-672aa

    Promoter

    tac

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCR2.1-Ins2-842

    Plasmid

    #53969

    Purpose

    842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

    Depositor

    Kevin Ferreri

    Article

    Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

    Insert

    mouse Ins2 gene partial (Ins2 Mouse)
    

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-10-3xFLAG-LGP2-K651E

    Plasmid

    #58685

    Purpose

    Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

    Insert

    LGP2-K651E (DHX58 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    mutation to CTD, K651E

    Promoter

    CMV

    Available Since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB

    Plasmid

    #193717

    Purpose

    Rapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Synthetic, Human)
    

    Tags

    2xmNeongreen-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid

    #193720

    Purpose

    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid

    #193719

    Purpose

    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Synthetic, Human)
    

    Tags

    2xHA-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBabe-Puro-IKBalpha-mut (super repressor)

    Plasmid

    #15291

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    inhibitor of Kappa B alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S32A/S36A: “super-repressor” allele (resistant to…

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. EGFP-BRCA1

    Plasmid

    #115491

    Purpose

    EGFP-BRCA1 expression vector. Parton clone BXY

    Depositor

    Rob Parton

    Article

    McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1009R (See depositor comments)

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCA528 CHMP1B 1-199

    Plasmid

    #115326

    Purpose

    Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGFP-N1 Full length Importin beta

    Plasmid

    #106941

    Purpose

    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

    Insert

    human Importin beta-1 (KPNB1) (KPNB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGEMHE-mScarlet-MAP4-MTBD

    Plasmid

    #171488

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Mutation

    Aminoacids 658-1126

    Promoter

    T7

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pRosa26-EF1a-hCas9IRESneo

    Plasmid

    #67987

    Purpose

    Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-hCas9IRESneopA
    

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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