We narrowed to 9,193 results for: Pol;

1. pGEMHE-miRFP-MAP4-MTBD

   Plasmid

   #171487

   Purpose

   T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   miRFP

   Mutation

   Aminoacids 658-1126

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX037

   Plasmid

   #167156

   Purpose

   MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   caffeic acid cycle genes
   

   Expression

   Plant

   Promoter

   pNOS, p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIPZ-bioGEF-UBwt-PURO

   Plasmid

   #208045

   Purpose

   Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   Ub (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-N-Myc_C223S USP7

   Plasmid

   #131243

   Purpose

   Mammalian expression of catalytically inactive Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains a point mutation that converts USP7 cyst…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv2-sgFXN-1

   Plasmid

   #246017

   Purpose

   All-in-one CRISPRko plasmid containing Cas9 and guide RNA targeting FXN

   Depositor

   Vamsi Mootha

   Article

   Ast et al Cell. 2019 May 30;177(6):1507-1521.e16.

   Insert

   Frataxin (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRv2-sgFXN-2

   Plasmid

   #246018

   Purpose

   All-in-one CRISPRko plasmid containing Cas9 and guide RNA targeting FXN

   Depositor

   Vamsi Mootha

   Article

   Ast et al Cell. 2019 May 30;177(6):1507-1521.e16.

   Insert

   Frataxin (FXN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRIG-Akt1

   Plasmid

   #53583

   Purpose

   lentiviral expression of constitutively active human Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Oct. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-T/O-Ds1-mCherry

   Plasmid

   #112855

   Purpose

   Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein

   Depositor

   David Sprinzak

   Article

   Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 1

   Insert

   DCHS1 (DCHS1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   doxycycline inducible promoter

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mScarlet-Giantin

    Plasmid

    #196872

    Purpose

    Expresion of Giantin-fused to mScarlet. Used as Golgi marker

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mScarlet-Giantin (GOLGB1 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPRv2_RB1#1

    Plasmid

    #174150

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 IRF7(5’UTR)-FF

    Plasmid

    #85490

    Purpose

    Firefly luciferase under the control of IRF7 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of IRF7 (Irf7 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. W118-1_hTGM1-Flag

    Plasmid

    #180406

    Purpose

    lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    TGM1 (TGM1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS2241

    Plasmid

    #111896

    Purpose

    ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple320 (POU4F1 Human)
    

    Use

    AAV

    Promoter

    Ple320 (POU4F1 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-Flag-Med25

    Plasmid

    #64771

    Purpose

    Mammalian protein expression of flag-tagged Med25

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25 (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-V5-hspB1

    Plasmid

    #63102

    Purpose

    Expression of HSPB1 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hGPR56-IRES-GFP

    Plasmid

    #52297

    Purpose

    Expresses human GPR56 and GFP in mammalian cells.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Human GPR56 coding sequence (ADGRG1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

    Plasmid

    #216478

    Purpose

    lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid

    #159390

    Purpose

    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

    Depositor

    Tarun Kapoor

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBabe-GFP-IKBalpha-mut (super repressor)

    Plasmid

    #15264

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEMS2273

    Plasmid

    #111899

    Purpose

    ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple343 (TUBB3 Human)
    

    Use

    AAV

    Promoter

    Ple343 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid

    #111389

    Purpose

    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    ChETA-mRuby2
    W3SL
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    W3SL is built from a shortened WPRE, an upstream …

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLVX-EF1α-BHLHE40-IRES-ZsGreen1

    Plasmid

    #153061

    Purpose

    Lentiviral vector expressing human BHLHE40

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET-Duet-1-NONO(53-312)

    Plasmid

    #135442

    Purpose

    Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Non-POU domain-containing octamer-binding protein (NONO Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.1+/-FLI1-C‐(K)‐DYK

    Plasmid

    #129497

    Purpose

    Mammalian expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. lentiCRISPRv2_sgCtrl#1

    Plasmid

    #174148

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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