We narrowed to 9,193 results for: Pol;

1. pET28_6xHis-PARP1-G972R

   Plasmid

   #174800

   Purpose

   Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G972R (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G972R, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG416GAL-DENV_NS3-GS-NS2B

   Plasmid

   #201940

   Purpose

   Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Article

   Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

   Insert

   DENV NS3-GS-NS2B (POLY )
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2274

   Plasmid

   #111900

   Purpose

   ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   Ple344 (TUBB3 Human)
   

   Use

   AAV

   Promoter

   Ple344 (TUBB3 MiniPromoter)

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTV001

   Plasmid

   #130265

   Purpose

   EatA expression plasmid containing 8His encoding region in permissive site of eatA gene

   Depositor

   James Fleckenstein

   Article

   Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do

   Insert

   eatA::His8
   

   Tags

   polyhistidine (internal)

   Expression

   Bacterial

   Mutation

   8His polyhistidine encoding region and XhoI site …

   Promoter

   araBAD

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACTB-(A)3x

   Plasmid

   #112057

   Purpose

   ACTB mRNA tagged with 3 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   3 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ACTB-(A)2x

   Plasmid

   #112056

   Purpose

   ACTB mRNA tagged with 2 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   2 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-EglN2 H358A-pBabe-puro

   Plasmid

   #22705

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated cDNA: point mutation in codon 358 changi…

   Available Since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME mKate2-dynactin1-811

   Plasmid

   #105971

   Purpose

   entry clone for gateway cloning, p150Glued subunit of dynactin, cofactor of dynein, dominant negative truncation, amino acids 1-811

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   dynactin1-811 (DCTN1 Human)
   

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   contains only amino acids 1-811

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-Flag-Avi-ires-CHD5

   Plasmid

   #68878

   Purpose

   Transient mammalian expression of CHD5 for BirA tagging

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5 (CHD5 Human)
   

   Tags

   FLAG/AVI

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pTWIN1-His6-Ssp-Httex1-29Q

    Plasmid

    #84350

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTWIN1-His6-Ssp-Httex1-37Q

    Plasmid

    #84351

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP044

    Plasmid

    #101168

    Purpose

    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTWIN1-His6-Ssp-Httex1-15Q

    Plasmid

    #84348

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag MFF iso5 S172A

    Plasmid

    #74382

    Purpose

    Flag tagged human S172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3 Flag MFF iso5 S155,172A

    Plasmid

    #74383

    Purpose

    Flag tagged human S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    See 155 Ala, Ser 172 Ala (numbering based on MFF …

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET41s-GST-ATF6a-AD

    Plasmid

    #64774

    Purpose

    bacterial expression of GST-tagged ATF6 (1-150)

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    ATF6a-AD (1-150) (ATF6 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    C-term deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pEMS2272

    Plasmid

    #111898

    Purpose

    ssAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple342 (TUBB3 Human)
    

    Use

    AAV

    Promoter

    Ple342 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX-GST-CHD5(Chromos)-FRAG

    Plasmid

    #68875

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only the two chromodomains of CHD5

    Promoter

    tac

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

22. Cherry-SRBC

    Plasmid

    #69492

    Purpose

    Expression of fluorescently tagged SRBC in mammalian cells

    Depositor

    Rob Parton

    Article

    Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

    Insert

    SRBC (Cavin3 Mouse)
    

    Tags

    Cherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA5-Flag-Med25-VWA

    Plasmid

    #64772

    Purpose

    Mammalian expression of Flag-tagged Med25-VWA

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    Med25-VWA (MED25 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C-terminal deletion

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCDNA3 Flag MFF iso5 S155A

    Plasmid

    #74381

    Purpose

    Flag tagged human S155A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 155 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pCMV-FLAG-CHD5-WT-331-672aa

    Plasmid

    #68870

    Purpose

    Mammalian expression of CHD5 histone binding domain (331-672aa)

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    331-672aa

    Promoter

    CMV

    Available Since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pEMS1136

    Plasmid

    #29056

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple198 (SLC6A4 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-hSyn1-DIO-eGFP

    Plasmid

    #227237

    Purpose

    Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

    Depositor

    Richard Huganir

    Article

    Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

    Insert

    eGFP (eGFP )
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMAPLe4 PCMTD1

    Plasmid

    #246503

    Purpose

    Recombinant protein expression of PCMTD1

    Depositor

    Steven Clarke

    Article

    Warmack et al Biochemistry. 2022 May 17;61(10):879-894

    Insert

    Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Residue 312 is Isoleucine in this plasmid but occ…

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-EF1A-DDHD2-30-202-Hygro

    Plasmid

    #238101

    Purpose

    Expression of structurally predicted WWE domain of DDHD2 with EGFP on C terminal

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    DDHD2 (DDHD2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 30-202 only

    Promoter

    EF1alpha

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-EF1A-TRIP12-391-938-EGFP-Hygro

    Plasmid

    #238092

    Purpose

    Expression of structurally predicted WWE domain of TRIP12 with EGFP on C terminal

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    TRIP12 (TRIP12 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 319-938 only

    Promoter

    EF1alpha

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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