We narrowed to 9,193 results for: Pol;

1. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

   Plasmid

   #191331

   Purpose

   Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
   

   Expression

   Mammalian

   Mutation

   DYNC1H1 motor domain aa 1453-4644

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV.CBA.YFP.miR-E_shIrak1-2

   Plasmid

   #180398

   Purpose

   Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shIrak1-2 (Irak1 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV.CBA.YFP.miR-E_shIrak1-3

   Plasmid

   #180399

   Purpose

   Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shIrak1-3 (Irak1 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV.CBA.YFP.miR-E_shIrak1-1

   Plasmid

   #180397

   Purpose

   Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   shIrak1-1 (Irak1 Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CBA

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX2T-TEV CHMP1B 4-178

   Plasmid

   #108284

   Purpose

   Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 179-199

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 CHMP1B 4-199

   Plasmid

   #115325

   Purpose

   Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-G871L

   Plasmid

   #174798

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-G871L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G871L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-A870L

   Plasmid

   #174797

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A870L (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A870L, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-K893I

    Plasmid

    #174799

    Purpose

    Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-K893I (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K893I, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKHH030_sgCtrl#2

    Plasmid

    #174143

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX026

    Plasmid

    #167153

    Purpose

    MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnLuz-ocsT
    

    Use

    Luciferase

    Tags

    His6-tag

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR_P4-P1R:PER36modp

    Plasmid

    #128431

    Purpose

    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    PEROXISADE36 (AT3G50990 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Mutation

    Premature stop codons introduced into the Xero1 (…

    Promoter

    PEROXISADE36

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pJC20cdc8.A18T

    Plasmid

    #99360

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJC20cdc8.E31K

    Plasmid

    #99361

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 31 to Lysine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJC20cdc8.110

    Plasmid

    #99363

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine and Glutamic acid 31 to L…

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-GST-CHD5-L518A/Y619E-FRAG

    Plasmid

    #68874

    Purpose

    IPTG inducible bacterial expression of CHD5 fragment

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5 (CHD5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    331-672aa, L518A/Y619E

    Promoter

    tac

    Available Since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. hKCNQ5(WT).ires.GFP-pcDNA3

    Plasmid

    #204360

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hKCNQ3(WT).ires.mCherry-pcDNA3

    Plasmid

    #204362

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hBACE1(D289N).ires.mCherry-pcDNA3

    Plasmid

    #204363

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    BACE1 (BACE1 Human)
    

    Expression

    Mammalian

    Mutation

    D289N

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. hKCNQ5(WT).ires.mScarlet-pcDNA3

    Plasmid

    #204361

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR223 SARS-CoV2 TEV-NSP12

    Plasmid

    #154410

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. hKCNQ5(K289N).ires.GFP-pcDNA3

    Plasmid

    #204364

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    K289N

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. hKCNQ5(P369T).ires.GFP-pcDNA3

    Plasmid

    #204365

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    P369T

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hKCNQ5(R443Q).ires.GFP-pcDNA3

    Plasmid

    #204366

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    R443Q

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hKCNQ5(H576R).ires.GFP-pcDNA3

    Plasmid

    #204367

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    H576R

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. hKCNQ5(V145G).ires.GFP-pcDNA3

    Plasmid

    #204368

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    V145G

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hKCNQ5(L341I).ires.GFP-pcDNA3

    Plasmid

    #204369

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    L341I

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. hKCNQ5(P369R).ires.GFP-pcDNA3

    Plasmid

    #204370

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    P369R

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hKCNQ5(S448I).ires.GFP-pcDNA3

    Plasmid

    #204371

    Purpose

    Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

    Depositor

    Aguan Wei

    Article

    Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

    Insert

    KCNQ5 (KCNQ5 Human)
    

    Expression

    Mammalian

    Mutation

    S448I

    Promoter

    CMV/T7 RNApol

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge