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We narrowed to 24,269 results for: fus

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Showing: 8841 - 8880 of 24269 results

  2. DsRed +9e

    Plasmid
    #191766
    Purpose
    Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e
    Depositor
    Daniel Gerlich
    Article
    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:
    Insert
    DsRed
    Tags
    N terminal fusion of positively charged polypetod…
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    191766-plasmid-map-sequence-id-383844

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  9. pONSY-Lifeact:mCherry

    Plasmid
    #111876
    Purpose
    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Lifeact fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111876-plasmid-map-sequence-id-213488

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  14. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  17. FN(9-10)-I27-CC

    Plasmid
    #85394
    Purpose
    MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Khalid Salaita
    Article
    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:
    Insert
    Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85394-plasmid-map-sequence-id-172698

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  18. pCALNL_chr13_102010574-102010650

    Plasmid
    #81213
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 13 (numbers in common name refers to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81213-plasmid-map-sequence-id-163268

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  21. pCALNL_Ch12_62418577-62418652

    Plasmid
    #81212
    Purpose
    pCALNL reporter contains two target sites consisting of PAM Cas9 site-gix psuedo site-Cas9 site-PAM that match region in centromeres of chromosomes 12 (numbers in common name refer to pos. in hg38)
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    gix-Neo-gix deletion cassette upstream of EGFP
    Expression
    Mammalian
    Promoter
    pCBa
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    81212-plasmid-map-sequence-id-163264

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  23. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  24. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

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  26. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  27. pCVL TAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50421
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Article
    Boissel et al Nucleic Acids Res. 2013 Nov 26.
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    50421-plasmid-map-sequence-id-79593

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  28. pMOD_B2000

    Plasmid
    #91059
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    none (begins with P2A to be fused to TALEN_1)
    Available Since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91059-plasmid-map-sequence-id-181870

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  29. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  37. Fc-E3

    Plasmid
    #181876
    Purpose
    Expresses murine Fc fusion to the first three EGF-like domains of human NOTCH3 WT
    Depositor
    Michael Wang
    Article
    Zhang et al Sci Rep. 2021 Aug 26;11(1):17246. doi: 1
    Insert
    Fc (murine) fused to NOTCH3 (human EGF-like domains 1-3) (NOTCH3 Mouse, Human)
    Tags
    Fc murine IgG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    181876-plasmid-map-sequence-id-357749

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  38. pA.CBh.TP-ZFP36L2-V5H6.MCh.Puro

    Plasmid
    #209951
    Purpose
    In mammalian cells expresses TP fused to a ZFP36L2 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    Zinc finger protein 36-like 2 (ZFP36L2 Human)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    TP (MS2 coat protein) and V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209951-plasmid-map-sequence-id-418732

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Showing: 8841 - 8880 of 24269 results