We narrowed to 7,925 results for: AMPH

1. pACYC-Cas12c2-noncoding

   Plasmid

   #120877

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12c2
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   constitutive

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC031 - RcrCas13a from Rhodobacter capsulatus R121

   Plasmid

   #91921

   Purpose

   Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   RcrCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEG R1-ChlorR-R4 #BGV049

   Plasmid

   #48943

   Purpose

   Contains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR4 sites for the creation of Destination vectors

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Gateway cloning vector

   Promoter

   na

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

   Plasmid

   #91917

   Purpose

   Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LweCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLaNGo11-1

   Plasmid

   #126053

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046940

   Plasmid

   #149261

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pDGE671

   Plasmid

   #153237

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   GFP

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMpGWB225

   Plasmid

   #68616

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Triple repeats of Citrine (3xCitrine)

   Expression

   Plant

   Promoter

   Cauliflower mosaic virus 35S promoter

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB527

    Plasmid

    #74869

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB525

    Plasmid

    #74867

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK3_020

    Plasmid

    #123735

    Purpose

    Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    ERT2-fkbp-spdCa9(205-1368)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRIME-TREX-GFP-FF3

    Plasmid

    #11667

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREG(R1-R3) #BGV141

    Plasmid

    #48958

    Purpose

    Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pOPO635

    Plasmid

    #195297

    Purpose

    pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABC operon of McCAST (Tn7575)
    

    Expression

    Bacterial

    Promoter

    IPTG inducible lac promoter

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid

    #202208

    Purpose

    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    

    Use

    Lentiviral

    Tags

    FLAG and LOV*

    Promoter

    Ef1a

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMpGWB411

    Plasmid

    #68676

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB429

    Plasmid

    #74823

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASP-SptP120-TD-HilA

    Plasmid

    #153330

    Purpose

    Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE151

    Plasmid

    #118501

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCASP-SptP167-3G124-HilA

    Plasmid

    #153322

    Purpose

    Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP167-3G124 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. JN607: pMVP/SB-DEST

    Plasmid

    #121860

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. IZ401: pMVP/Lenti/Neo-DEST

    Plasmid

    #121850

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSK234

    Plasmid

    #131149

    Purpose

    dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Type

    Empty backbone

    Use

    Gateway destination vector

    Expression

    Yeast

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. KTK_136

    Plasmid

    #180520

    Purpose

    Empty backbone for D1.1 cloning, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGBW-m4046925

    Plasmid

    #145694

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. J23101

    Plasmid

    #55176

    Purpose

    Very weak constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    J23101 promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    J23101

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCASP-SptP120-3m160-HilA

    Plasmid

    #153325

    Purpose

    Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3m160 DARPin
    

    Use

    Salmonella expression

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBTK130

    Plasmid

    #110584

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    T7 promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. KK402: pMVP/Zeo-DEST

    Plasmid

    #121859

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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