We narrowed to 9,302 results for: Coli

1. pAIP4_4O6G

   Plasmid

   #234175

   Purpose

   Heterologous protein expression of uncharacterized protein in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4O6G
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pAIP4_3K1H

   Plasmid

   #234157

   Purpose

   Heterologous protein expression of O25709_HELPY in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3K1H
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. MZB184

   Plasmid

   #228488

   Purpose

   Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Type

   Empty backbone

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MZB223

   Plasmid

   #228491

   Purpose

   Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Type

   Empty backbone

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJBL7042

   Plasmid

   #167213

   Purpose

   overexpression of MerR in E. coli for cell-free mercury sensing

   Depositor

   Julius Lucks

   Insert

   MerR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJBL7043

   Plasmid

   #167214

   Purpose

   overexpression of ArsR in E. coli for cell-free arsenic sensing

   Depositor

   Julius Lucks

   Insert

   ArsR EP3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJBL7047

   Plasmid

   #167217

   Purpose

   overexpression of CadR in E. coli for cell-free cadmium sensing

   Depositor

   Julius Lucks

   Insert

   CadR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOP5TT

   Plasmid

   #112613

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His-thioredoxin and Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOP5BP

   Plasmid

   #112608

   Purpose

   Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His-GB1 and Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28b(+)-TM0848

    Plasmid

    #67287

    Purpose

    Expresses TM0848 in E. coli

    Depositor

    SeWon Suh

    Insert

    GTP-binding protein YqeH
    

    Use

    Tags

    N tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pOpen-ECOpolA

    Plasmid

    #165532

    Purpose

    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    E. coli DNA Polymerase I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pExp-CHis

    Plasmid

    #112559

    Purpose

    Used for expression of protein in E. coli as C-terminual 8His fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAIP4_2H7W

    Plasmid

    #234220

    Purpose

    Heterologous protein expression of chagasin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAIP4_2NT3

    Plasmid

    #234208

    Purpose

    Heterologous protein expression of response regulator homolog in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2NT3
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCK341

    Plasmid

    #192640

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dSpRY
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    SoxS has R93A and S101A mutations and dSpRY has s…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSecUAG-Evol2

    Plasmid

    #163148

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons

    Depositor

    Dieter Söll

    Article

    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

    Inserts

    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG), Cys…

    Promoter

    EM7, araBAD, native As SelD promoter, and native …

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLgw V5-EcoDam

    Plasmid

    #59210

    Purpose

    Mammalian DamID control lentiviral vector for expression of V5-Dam only

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Insert

    EcoDam
    

    Use

    Lentiviral; Damid

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter and heat shock minima…

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pORTMAGE-1

    Plasmid

    #72680

    Purpose

    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    lower strength RBS before mutL E32K allele than i…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only