We narrowed to 7,926 results for: AMPH

1. pDGE502

   Plasmid

   #153275

   Purpose

   M8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. C335

   Plasmid

   #141125

   Purpose

   Expresses chimeric repressor (RbsR-L) under Cin promoter

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

   Inserts

   LLhR
   AiiA (AQ980_RS12585 Synthetic)
   

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Chimera: DNA binding domain of LacI and ligand bi…

   Promoter

   engineered cin promoter (responds to C14-HSL) and…

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SMT201

   Plasmid

   #134816

   Purpose

   SMT101 with TET promoter for TYMS, in vivo assays

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7 and TET

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCASP-SptP35-TD-HilA

   Plasmid

   #153332

   Purpose

   Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   HA4-7c12 Tandem Monobody
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCASP-SptP92-TD-HilA

   Plasmid

   #153331

   Purpose

   Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP92-HA4-7c12 Tandem Monobody
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASP-3G124-HilA

   Plasmid

   #153324

   Purpose

   Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   3G124 DARPin but no expression
   

   Use

   Salmonella expression

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046774

   Plasmid

   #145574

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGBW-m4046846

   Plasmid

   #145575

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MYMH 351

   Plasmid

   #138058

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-10AG 11AC-GFP
   

   Expression

   Bacterial

   Mutation

   AAA GAG GAG GCA

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. MYMH 336

    Plasmid

    #138057

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    GCA GAG GAG ACA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MYMH 333

    Plasmid

    #138056

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    GAA GAG GAG ACA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MYMH 330

    Plasmid

    #138055

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb- 2AC 11AC-GFP
    

    Expression

    Bacterial

    Mutation

    ACA GAG GAG ACA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MYMH 327

    Plasmid

    #138054

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC-GFP
    

    Expression

    Bacterial

    Mutation

    GCA GAG GAG AAA

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK110

    Plasmid

    #110576

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP6 Promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBTK112

    Plasmid

    #110577

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP12b Promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBTK113

    Plasmid

    #110578

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP32 Promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. MTK234_005

    Plasmid

    #123992

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA homology arm-1)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK234_006

    Plasmid

    #123993

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA Homology arm-2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_007

    Plasmid

    #123994

    Purpose

    Encodes the spCas9 sgRNA1 cROSA26 (homology arm 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA for cROSA Homology arm-3
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK234_056

    Plasmid

    #123921

    Purpose

    Encodes the spCas9 gRNA GFP dropout expression cassette (human u6 promoter and constant region 3) as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    gRNA-GFPDROPOUT-hu6_20N_cr3
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. MTK234_026

    Plasmid

    #123906

    Purpose

    Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    human non targeting sgRNA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MTK234_028

    Plasmid

    #123908

    Purpose

    Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA2 -hThal5.10-2_hg18
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MTK234_029

    Plasmid

    #123909

    Purpose

    Encodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA3 -hThal5.10-2_hg18
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MTK6_013

    Plasmid

    #123867

    Purpose

    Encodes Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) expressing a Blasticidin resistance cassette without a terminator to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    WPRE-Blast (no terminator)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. MTK3_018

    Plasmid

    #123733

    Purpose

    Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    spdCas9(1-204)-frb-D10A-ERT2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSTAP-seq_fly-4xUAS

    Plasmid

    #125149

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. JK305: pMVP/Blast-DEST

    Plasmid

    #121855

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. JL104: pMVP/Neo-DEST

    Plasmid

    #121857

    Purpose

    pMVP destination vector, empty expression vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKD250.2

    Plasmid

    #125077

    Purpose

    Expression of torR under PLtetO-1, output promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    torR (torR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSR300.3

    Plasmid

    #125079

    Purpose

    Expression of uhpA under PLtetO-1, output Promoter PuhpT99

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    uhpA (uhpA )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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