We narrowed to 9,194 results for: Pol;

1. FUW-OSKM

   Plasmid

   #20328

   Purpose

   Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

   Depositor

   Rudolf Jaenisch

   Article

   Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

   Plasmid

   #213024

   Purpose

   Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

   Depositor

   Martin S. Taylor, Oliver Weichenrieder

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (238-1061)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Promoter

   T7 / Lac

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

   Plasmid

   #61364

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tetO-ALN

   Plasmid

   #43918

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B, NR4A2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Gylcine-serine-glycine (GSG) linker followed by v…

   Promoter

   TRE promoter, Tet-ON

   Available Since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pWZL Neo Myr Flag FGFR1

   Plasmid

   #20486

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   FGFR1 (FGFR1 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINB-M1-di-Ub(G76V)-HOIL-1

   Plasmid

   #229539

   Purpose

   Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli

   Depositor

   Bernhard Lechtenberg

   Article

   Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025

   Inserts

   Ubiquitin (UBC Human)
   Ubiquitin (UBC Human)
   HOIL-1 (RBCK1 Human)
   

   Tags

   6xHis-3C

   Expression

   Bacterial

   Mutation

   Changed Gly 76 to Val and changed Gly 76 to Val

   Promoter

   T7

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWZL Neo Myr Flag GCK

   Plasmid

   #20492

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   GCK (GCK Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pWZL Neo Myr Flag AKT3

   Plasmid

   #20423

   Depositor

   William Hahn, Jean Zhao

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   AKT3 (AKT3 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. MSCV-N JCV-cy ER

   Plasmid

   #37866

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   JCV CY ER (Jvgp5, Jvgp6 JC Polyomavirus)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV-N JCV mad1 ER

    Plasmid

    #37865

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    JCV mad1 ER (Jvgp5, Jvgp6 JC Polyomavirus)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV-N HPyV7

    Plasmid

    #37869

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPyV7 ER (HPyV7_gp4, HPyV7_gp5 Polyomavirus HPyV7)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

12. MSCV-N HPyV6

    Plasmid

    #37868

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPyV6 ER (HPyV6_gp4, HPyV6_gp5 Polyomavirus HPyV6)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. MSCV-N WU ER

    Plasmid

    #37867

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    WU ER (WUPyV_gp5, WUPyV_gp4 WU polyomavirus)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pLENTI EF1alpha FLAG/HA-LCOR

    Plasmid

    #114444

    Purpose

    Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

    Depositor

    Adrian Bracken

    Article

    Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

    Insert

    LCOR (LCOR Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn1-DIO-SEP-GluA2Q

    Plasmid

    #227234

    Purpose

    Large cargo capacity AAV that expresses SEP tagged AMPA receptor subunit GluA2 R607Q mutant in neurons expressing Cre recombinase

    Depositor

    Richard Huganir

    Article

    Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

    Insert

    SEP-GluA2 (Gria2 )
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Q607R

    Promoter

    hSyn1

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn1-DIO-SEP-GluA1R

    Plasmid

    #227236

    Purpose

    Large cargo capacity AAV that expresses SEP tagged AMPA receptor subunit GluA1 Q582R mutant in neurons expressing Cre recombinase

    Depositor

    Richard Huganir

    Article

    Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

    Insert

    SEP-GluA1 Q582R (Gria1 Rat)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Q582R

    Promoter

    hSyn1

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC18_BCR_GUSB_e19a2

    Plasmid

    #190884

    Purpose

    molecular monitoring of atypical BCR::ABL1 fusion transcripts

    Depositor

    Nicholas Cross

    Article

    Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

    Inserts

    e19a2 BCR::ABL1 fusion cDNA
    GUSB cDNA (GUSB Human)
    BCR cDNA (BCR Human)
    

    Use

    Cloned cdna transcripts for use in rt-qpcr bcr::a…

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC18_BCR_GUSB_e6a2

    Plasmid

    #190882

    Purpose

    molecular monitoring of atypical BCR::ABL1 fusion transcripts (e6a2)

    Depositor

    Nicholas Cross

    Article

    Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

    Inserts

    e6a2 cDNA
    GUSB cDNA transcript (GUSB )
    BCR cDNA (BCR Human)
    

    Use

    Cloned cdna transcripts for use in rt-qpcr bcr::a…

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC18_BCR_GUSB_e8a2

    Plasmid

    #190883

    Purpose

    molecular monitoring of atypical BCR::ABL1 fusion transcripts

    Depositor

    Nicholas Cross

    Article

    Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

    Inserts

    e8a2 BCR::ABL1 fusion cDNA
    GUSB cDNA (GUSB Human)
    BCR cDNA (BCR Human)
    

    Use

    Cloned cdna transcripts for use in rt-qpcr bcr::a…

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid

    #213026

    Purpose

    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid

    #213030

    Purpose

    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid

    #213031

    Purpose

    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

    Depositor

    Donna Romero, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    

    Use

    Luciferase

    Tags

    Firefly luciferase antisense intron (3' UTR)

    Expression

    Mammalian

    Promoter

    pTRE bidirectional tet-on

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FUW-SOKM

    Plasmid

    #20325

    Purpose

    Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213027

    Purpose

    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    D702Y (RT- reverse transcriptase catalytic dead)

    Promoter

    CMV

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC18_BCR_GUSB_ABL1_e14a3

    Plasmid

    #190885

    Purpose

    molecular monitoring of atypical BCR::ABL1 fusion transcripts

    Depositor

    Nicholas Cross

    Article

    Schafer et al J Cancer Res Clin Oncol. 2021 Oct;147(10

    Inserts

    e14a3 BCR::ABL1 fusion transcript
    ABL1 cDNA (ABL1 Human)
    GUSB cDNA (GUSB Human)
    BCR (BCR Human)
    

    Use

    Cloned cdna transcripts for use in rt-qpcr bcr::a…

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSrTA1662-toolkit

    Plasmid

    #154372

    Purpose

    Expresses SrTA1662 in plants

    Depositor

    Brande Wulff

    Article

    Gaurav et al Nat Biotechnol. 2022 Mar;40(3):422-431.

    Insert

    SrTA1662
    

    Expression

    Bacterial and Plant

    Promoter

    Native promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223 SARS-CoV-2 NSP12_nostop

    Plasmid

    #149314

    Purpose

    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge