We narrowed to 17,699 results for: SHE

1. 8GCY

   Plasmid

   #214440

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBLB:PBC020-G02:C248501 (CBLB Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 8DYS

   Plasmid

   #214439

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EIF2A:PBC022-C06:C245159 (EIF2A Human)
   

   Use

   Baculovirus

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   Feb. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET28-mEGFP(A206K)-TwStrep

   Plasmid

   #208622

   Purpose

   for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone

   Depositor

   Quanfu Ma

   Insert

   mEGFP(A206K)
   

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Mutation

   (compared with pEGFP-C1, protein level) A206K

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGB-05-06-GST-ATG14

   Plasmid

   #187987

   Purpose

   Plasmid for the protein expression of GST-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1321

   Depositor

   Sascha Martens

   Insert

   ATG14 (ATG14 Human)
   

   Tags

   GST

   Expression

   Bacterial and Insect

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNC-Green

   Plasmid

   #193394

   Purpose

   Nimble cloning-compatible vector for plant ectopic expression

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNC-Green0029

   Plasmid

   #193396

   Purpose

   Nimble cloning-compatible vector for plant ectopic expression

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNC-HSE401

   Plasmid

   #193434

   Purpose

   Nimble cloning-compatible vector for genome editing in plants

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a-DIO-somAion-citrine

   Plasmid

   #192573

   Purpose

   AAV-vector for optogenetic long-term silencing of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somAion
   

   Use

   AAV

   Tags

   Citrine - soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   Ef1-alpha

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB6-Hipp11-td-sfGFP-cre reporter-3pA stop-FNF-DTA

   Plasmid

   #183026

   Purpose

   B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   td-sfGFP
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB6-Hipp11-tdTomato-cre reporter-3pA stop-FNF-DTA

    Plasmid

    #183025

    Purpose

    B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB6-Hipp11-tdTomato cre reporter-FNF-DTA

    Plasmid

    #183024

    Purpose

    B6J Hipp11-pCAG-loxP-FNF-bGHpA-3xSV40pA-loxP-tdTomato-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MDKA-c014

    Plasmid

    #175464

    Purpose

    Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.

    Depositor

    Opher Gileadi

    Insert

    MDK (MDK Human)
    

    Tags

    HRV 3C-Fc-His6 and PTPRS (Gg) SP

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. EPB41L3A-c021

    Plasmid

    #175460

    Purpose

    Protein expression in bacterial cells. FERM domain, P107-L390. Used for crystallography (PDB:6IBE).

    Depositor

    Opher Gileadi

    Insert

    EPB41L3 (EPB41L3 Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid

    #159906

    Purpose

    Mutagenesis of Dcc

    Depositor

    Larry Zweifel

    Insert

    Dcc (Dcc Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-Cv3

    Plasmid

    #99351

    Purpose

    Synthetic sequence for single-stranded DNA production

    Depositor

    Mark Bathe

    Article

    Veneziano et al Sci Rep. 2018 Apr 25;8(1):6548. doi: 10.

    Insert

    Line from The Crucible Act 3
    

    Expression

    Bacterial

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDK362

    Plasmid

    #134194

    Purpose

    Retroviral plasmid with SKAP insert

    Depositor

    Iain Cheeseman

    Insert

    SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
    

    Use

    Retroviral

    Tags

    LAP (GFP)

    Expression

    Mammalian

    Mutation

    aa 166-316 with G5 and Jens siRNA hardening

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVd2-Dr-TrkB

    Plasmid

    #127955

    Purpose

    Myr DrTrkA expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

    Insert

    Myr-3xSAG-Dr-hel4-TrkB
    

    Expression

    Mammalian

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ad-EEF1D-S133E

    Plasmid

    #131577

    Purpose

    Expresses EEF1D with S133E mutation in mammalian cells

    Depositor

    Haiyan Xu

    Insert

    Eukaryotic translation elongation factor 1 delta (EEF1D Human)
    

    Use

    Adenoviral

    Tags

    No

    Mutation

    changed Serine 133 to Glutamic acid

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103 MAGI1 WW domain #1

    Plasmid

    #104242

    Purpose

    Bacterial expression of WW domain #1 from MAGI1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    MAGI1 WW domain #1 (MAGI1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103 MAGI1 WW domain #2

    Plasmid

    #104243

    Purpose

    Bacterial expression of WW domain #2 from MAGI1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    MAGI1 WW domain #2 (MAGI1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid

    #87154

    Purpose

    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJK606

    Plasmid

    #72529

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subunit with C-terminal His6 tag (AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK500

    Plasmid

    #72245

    Purpose

    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and endogenous

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJK624

    Plasmid

    #72539

    Purpose

    Produces Acetobacter aceti 1023 OrfY; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit with C-terminal His6 tag (AaOrfY, AaPurQ, AaPurLH6)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK625

    Plasmid

    #72541

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 4QQI

    Plasmid

    #61949

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ANKRA:JMC023-D07:C211743 (ANKRA2 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. 2MJ8

    Plasmid

    #61956

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CDYL2 (CDYL2 Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pT7-Venus-cdc25C5

    Plasmid

    #40969

    Depositor

    Olivier Gavet

    Insert

    cdc25C5 (CDC25C Human)
    

    Use

    In vitro transcription

    Tags

    Venus

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

29. 2NR8

    Plasmid

    #60108

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KIF9:kif9_dml-X01:C200670 (KIF9 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. 3HX4

    Plasmid

    #60137

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TgCDPK4:MAC03F-A01:C208088
    

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits