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LentiCRISPR-B_2xsgRNAs_RBM7 (pAVA3250)

Plasmid

#239322

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting RBM7

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting RBM7 (RBM7 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC10 (pAVA3318)

Plasmid

#239295

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC10

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC10 (EXOSC10 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)

Plasmid

#239296

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting C1D (C1D Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC2 (pP18(T)_C3-AVA4067)

Plasmid

#239301

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC2

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC2 (EXOSC2 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC4 (pP18(T)_C11-AVA4065)

Plasmid

#239302

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC4

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC4 (EXOSC4 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC5 (pP18(T)_B12-AVA4068)

Plasmid

#239303

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC5

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC5 (EXOSC5 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-2-EF1a-LibVec

Plasmid

#239593

Purpose

expresses non-targeting (NT) guide#2

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgNT-1-EF1a-LibVec

Plasmid

#239592

Purpose

expresses non-targeting (NT) guide#1

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

NT (non-targeting guide)

Use

AAV; To deliver a non-targeting guide (serving as…

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-CD20-Puro

Plasmid

#209755

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pITGA5_1745G>U

Plasmid

#173812

Purpose

mir218 binding site2 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT1745 ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_473G>A

Plasmid

#173811

Purpose

mir218 binding site1 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT473 ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_Q853L

Plasmid

#174008

Purpose

Fibronectin receptor binding site point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

FibronR ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2462

Plasmid

#97064

Purpose

Expressing Sulfolobus solfataricus orf SSO2462 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2462

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2452

Plasmid

#97059

Purpose

Expressing Sulfolobus solfataricus orf SSO2452 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2452

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF126

Plasmid

#97021

Purpose

Expressing Pyrobaculum spherical virus orf126 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 126

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA5006

Plasmid

#97016

Purpose

Expressing Pseudomonas aeruginosa orf PA5006 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA5006

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA0856

Plasmid

#97014

Purpose

Expressing Pseudomonas aeruginosa orf PA0856 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA0856

Tags

N-ter TEV cleavable 6HIs

Mutation

N-terminal deletion (AA 1-34)

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4631

Plasmid

#97017

Purpose

Expressing Pseudomonas aeruginosa orf PA4631 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Nucleoside-diphosphate-sugar epimerase

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2373

Plasmid

#97058

Purpose

Expressing Sulfolobus solfataricus orf SSO2373 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2373

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEHISSSO1986

Plasmid

#97057

Purpose

Expressing Sulfolobus solfataricus orf SSO1986 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1986

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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