We narrowed to 10,393 results for: plasmids 101

1. pDONR223_TRAP1_WT_V5

   Plasmid

   #82994

   Purpose

   Gateway Donor vector containing TRAP1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   TRAP1 (TRAP1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_BMP2_WT

   Plasmid

   #82101

   Purpose

   Gateway Donor vector containing BMP2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   BMP2 (BMP2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23-hs737

   Plasmid

   #222472

   Purpose

   Luciferase vector containing the hs737 enhancer sequence (reference sequence).

   Depositor

   Tychele Turner

   Article

   Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

   Insert

   hs737 enhancer (reference sequence) (LOC110120928 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201819

   Purpose

   A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Expression

   Mammalian

   Promoter

   pOTTC1751

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEP4 E02S ET2K

   Plasmid

   #20927

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
   

   Use

   Episomal/ebna

   Available Since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

   Plasmid

   #235086

   Purpose

   GFP splicing reporter with CORO1B intron

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   custom bidirectional

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

   Plasmid

   #102421

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 (Tet1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Modified at the Dharmacon SMARTpool siRNA #2 targ…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav1.2 Ca2+ channel scFv [N263/31]

   Plasmid

   #182083

   Purpose

   Mammalian Expression of Cav1.2 Ca2+ channel scFv. Derived from hybridoma N263/31.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Cav1.2 Ca2+ channel (Rattus norvegicus) (Cacna1c Mouse)
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Kv1.1 K+ channel scFv [K20/78]

    Plasmid

    #182065

    Purpose

    Mammalian Expression of Kv1.1 K+ channel scFv. Derived from hybridoma K20/78.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.1 K+ channel (Rattus norvegicus) (Kcna1 Mouse)
    

    Tags

    Sortase, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_SpCas9_USP7 Exon 3 gRNA

    Plasmid

    #131257

    Purpose

    pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    humanised S. pyogenes Cas9 nuclease
    

    Expression

    Mammalian

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB533_15F11-sfGFP

    Plasmid

    #167526

    Purpose

    H4K20me1-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

    Insert

    15F11-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV2_HLF-ZE

    Plasmid

    #175034

    Purpose

    HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

    Depositor

    Guy Sauvageau

    Article

    Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

    Insert

    HLF ires ZsGreen P2A tEGFR
    

    Use

    AAV

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV2_HLF-ZP

    Plasmid

    #175035

    Purpose

    HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

    Depositor

    Guy Sauvageau

    Article

    Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

    Insert

    HLF ires ZsGreen P2A Puro
    

    Use

    AAV

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-ADP-MCS-FLAG

    Plasmid

    #192360

    Purpose

    Adipocyte-specific overexpression of genes

    Depositor

    Yifu Qiu

    Article

    Xue et al Cell Metab. 2022 Sep 6;34(9):1325-1341.e

    Type

    Empty backbone

    Use

    AAV

    Tags

    3xflag

    Expression

    Mammalian

    Promoter

    adiponectin promoter

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV[FLEX]-CAG-EGFP-Ctrl-sponge

    Plasmid

    #235152

    Purpose

    Expresses a EGFP-based control sponge in a Cre recombinase-dependent manner

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    EGFP/control sponge
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 Flag Pu.1

    Plasmid

    #66974

    Purpose

    Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Spi1 (Spi1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p15a_AcCas12n

    Plasmid

    #203806

    Purpose

    p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12n
    

    Use

    CRISPR

    Promoter

    rpsl

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ141D-peg

    Plasmid

    #141081

    Purpose

    Gateway compatible prime editing gRNA empty backbone vector

    Depositor

    Yiping Qi

    Article

    Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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