We narrowed to 32,004 results for: Eng

1. AAVS1-Puro CAG PSMA nanobody T-CAR

   Plasmid

   #171962

   Purpose

   Donor plasmid for PSMA nanobody T-CAR expression in human cells

   Depositor

   Xiaoping Bao

   Article

   Harris et al J Immunol Regen Med. 2023 May;20:100074.

   Insert

   Synthetic anti-PSMA nanobody CAR
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Oct4-IRES-eGFP-PGK-Neo

   Plasmid

   #48681

   Purpose

   For knock-in donor vector by CRISPR/CAS system

   Depositor

   Rudolf Jaenisch

   Article

   Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6xHsa.NFKB:d2EGFP

   Plasmid

   #239990

   Purpose

   Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

   Depositor

   Raquel Espin-Palazon

   Insert

   Destabilized EGFP
   

   Promoter

   cfos minimal promoter

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDIRECT_25H

   Plasmid

   #91145

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDIRECT_21C

   Plasmid

   #91130

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ad-Flag-XBP1s

   Plasmid

   #63678

   Purpose

   Adenoviral vector for XBP1s expression

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-dDIO-vCre

   Plasmid

   #118029

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-dDIO-vCre
   

   Use

   AAV

   Mutation

   See depositor comments below

   Promoter

   TRE

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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