We narrowed to 32,034 results for: Eng

1. pBTK601

   Plasmid

   #110607

   Purpose

   BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   Cas9
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

   Plasmid

   #136381

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-TV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDIRECT_25G

   Plasmid

   #91144

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDIRECT_25I

   Plasmid

   #91146

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

   Plasmid

   #127775

   Purpose

   Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   sox10 (SOX10 Chicken)
   

   Use

   Enhancer reporter construct

   Tags

   Avi

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDIRECT_22D

   Plasmid

   #91136

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNTPTP alpha (S180A/S204A)

   Plasmid

   #17700

   Depositor

   Xinmin Zheng

   Article

   Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

   Insert

   PTPalpha S180A S204A (PTPRA Human)
   

   Expression

   Mammalian

   Mutation

   S180A / S204A

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pDIRECT_21D

   Plasmid

   #91131

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NG1237 pCI-TPI-PTC160-4H

   Plasmid

   #65804

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Tags

   4H (4 copies of short beta-globin 3'UTR for …

   Expression

   Mammalian

   Mutation

   premature stop codon at 160

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJY-RpABE-FT_INF

    Plasmid

    #112874

    Purpose

    binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting FT
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge