We narrowed to 15,892 results for: NOL
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Plasmid#73295PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-Af-mut
Plasmid#120829PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5 V114M/V872M - "african mut"
TagsV5ExpressionMammalianMutationV114M/V872MPromoterCMVAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)
Plasmid#160610PurposeTranscriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:AsCas12a:T35s
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Vav3-SH2
Plasmid#46530DepositorInsertVav3 (Vav3 Mouse)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 660-774PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd
Plasmid#182149PurposeTo insert attP-fwd attachment site at IDS2 in human cells via twinPEDepositorInsertIDS2-A4b-attP-fwd pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-IDS_DF_D1b_attB_rev
Plasmid#182152PurposeTo insert attB-rev attachment site at IDS in human cells via twinPEDepositorInsertIDS-D1b-attB-rev pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd
Plasmid#182150PurposeTo install attP-fwd attachment site at IDS2 in human cells via twinPEDepositorInsertIDS2-B7b-attP-fwd pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB3049(gRNA XII-4)
Plasmid#73291PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Tenc1-SH2
Plasmid#46524DepositorInsertTenc1 (TNS2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1114-1264PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG
Plasmid#127308PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127316PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs5-SH2
Plasmid#46508DepositorInsertSocs5 (SOCS5 Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 378-481PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)
Plasmid#160635PurposeTU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminatorDepositorInsertAcrIIA4
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grap-SH2
Plasmid#46434DepositorInsertGrap (GRAP Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 51-160PromoterTacAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST
Plasmid#127318PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and CL1/PESTExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE0ΔiNLS
Plasmid#107684PurposeExpresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cellsDepositorInsertdLbCpf1-BE0ΔiNLS
ExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCfB3038(XII-1 MarkerFree)
Plasmid#73278PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3040(XII-4 MarkerFree)
Plasmid#73280PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger1-TS-YFP)
Plasmid#127245PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger1-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCAGAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger3-TS-YFP)
Plasmid#127242PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger3-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCAGAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterU6-26Available SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Cten-SH2
Plasmid#46421DepositorInsertCten (TNS4 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 444-570PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1-gfp
Plasmid#48133PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2A1
Plasmid#67076PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW1997_pExpr-hU6-CRISPR-DECODER
Plasmid#87549PurposeExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinasesDepositorInsertgRNAs targeting NGN2, MIAT, ACTC1, TTN
UseCRISPR, Cre/Lox, and Synthetic BiologyExpressionMammalianAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABE8e(TadA-8e V82G)
Plasmid#138494PurposeA-to-G base editorDepositorInsertecTadA(8e V82G)-nSpCas9
ExpressionMammalianMutationTadA-8e V82GAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat2-SH2
Plasmid#46512DepositorInsertStat2 (STAT2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 566-681PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only