We narrowed to 15,892 results for: NOL

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pCAGEN_NES_V5_eMagAF_C74

Plasmid

#235615

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_eMagBF_N73

Plasmid

#235635

Purpose

Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

NES-V5-eMagBF

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_N167_eMagBF_V5_NES

Plasmid

#235622

Purpose

Encodes ALIBi N-terminal fragment, residues 1-167, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-167

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N195_eMagBF_V5_NES

Plasmid

#235624

Purpose

Encodes ALIBi N-terminal fragment, residues 1-195, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-195

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMT2092

Plasmid

#228342

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2093

Plasmid

#228343

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2094

Plasmid

#228344

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2095

Plasmid

#228345

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2096

Plasmid

#228346

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2076

Plasmid

#228337

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT2077

Plasmid

#228338

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT1434

Plasmid

#228317

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT949

Plasmid

#228289

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT951

Plasmid

#228291

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKN108

Plasmid

#228284

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.BC(p1-10)-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231355

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

Plasmid

#231347

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pscAAV.BC(p1-6)-CAG-EGFP-SV40pA-BC(p1-6)

Plasmid

#231349

Purpose

Self complementary AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4635

Plasmid

#215263

Purpose

Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-miniCGBE1

Plasmid

#198548

Purpose

Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-miniCGBE1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NRRH

Plasmid

#198540

Purpose

Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRRH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NRCH

Plasmid

#198542

Purpose

Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYAMTr2GCsgSc-SeLEU2

Plasmid

#224871

Purpose

Disruption of LEU2 genes in the Saccharomyces sense strict group species

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

Plasmid

#225438

Purpose

Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-FRT-OCS-BeYDVRepRepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

Plasmid

#225436

Purpose

BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

BeYDV geminino 1.0

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

Plasmid

#225437

Purpose

Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-att-OCS-BeYDVRep/RepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

Plasmid

#225430

Purpose

OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-FRT-OCSterm-FRT-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

Plasmid

#225431

Purpose

OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-att-OCSterm-att-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)

Plasmid

#225439

Purpose

Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

HygR-MAR10-gemiGFP-MAR10

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GB4636

Plasmid

#215264

Purpose

Module for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

Etr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge