We narrowed to 14,028 results for: RING

1. pCMV-haA3A-CBE-A-NG

   Plasmid

   #220895

   Purpose

   Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

   Depositor

   Dali Li

   Article

   Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

   Insert

   haA3A-CBE-A-NG
   

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-His:PYL1:nls:VPR

   Plasmid

   #135988

   Purpose

   Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   PYL1 domain fused to the VPR activation domain
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

   Plasmid

   #32475

   Purpose

   Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAMQ79G,Q139G-5HT3HC
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   IRES-EGFP

   Promoter

   Synapsin

   Available Since

   Dec. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and EF1-a

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-BG505.SOSIP-8his

   Plasmid

   #111847

   Purpose

   Mammalian expression plasmid for soluble BG505 SOSIP.664

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   8his purification tag and CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159857

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAGGS-EGFP-NLS-3XFLAG-V5

   Plasmid

   #196090

   Purpose

   Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   GFP
   

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Promoter

   CAGGs

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ChRger2-TS-EYFP

   Plasmid

   #127237

   Purpose

   High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   ChRger2-TS-EYFP
   

   Use

   AAV

   Tags

   EYFP and SpyTag

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSF-p15A-trc- YFP

   Plasmid

   #186569

   Purpose

   p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

   Depositor

   Teresa de Diego Puente

   Article

   Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

   Insert

   YFP
   

   Expression

   Bacterial

   Promoter

   trc promoter

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PA-Dre

    Plasmid

    #169706

    Purpose

    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag-T2A-pMag-Dre247
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PME-1 pcDNA-StrepIII

    Plasmid

    #119292

    Purpose

    Mammalian overexpression of StrepII-tagged full length PPME1

    Depositor

    Jukka Westermarck

    Article

    Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

    Insert

    PPME1 (PPME1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIV-EF1a-2A-ZsGreen

    Plasmid

    #231985

    Purpose

    Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    2A-ZsGreen
    

    Use

    Lentiviral

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid

    #83239

    Purpose

    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCpdCr
    

    Tags

    roGFP2 fusion with Tsa2dCpdCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBEI-6411

    Plasmid

    #47050

    Purpose

    BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
    

    Use

    Synthetic Biology; Bglbrick

    Expression

    Bacterial

    Promoter

    Pbad

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. hGAPDH-rLuc

    Plasmid

    #82479

    Purpose

    reporter of GAPDH expression

    Depositor

    Albee Messing

    Article

    Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

    Insert

    GAPDH promoter (GAPDH Human)
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    hGAPDH

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHLuc (Antares-SEPLuc C1A4E+6)

    Plasmid

    #141089

    Purpose

    final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-cofilin WT

    Plasmid

    #78295

    Purpose

    Mammalian expression of mouse cofilin fused to EGFP

    Depositor

    Michael Frotscher

    Article

    Chai et al Development. 2016 Mar 15;143(6):1029-40.

    Insert

    cofilin 1 (Cfl1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

    Plasmid

    #84472

    Purpose

    pAAV-RAM-ArchT

    Depositor

    Yingxi Lin

    Article

    Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

    Insert

    ArchT
    

    Use

    AAV

    Tags

    EGFP

    Promoter

    TRE

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. LVDP-CArG-RE-GPR

    Plasmid

    #89762

    Purpose

    Quantitative measurement of CArG response element activity.

    Depositor

    Stelios Andreadis

    Article

    Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

    Insert

    6X CArG-RE
    

    Use

    Lentiviral

    Tags

    CMV-ZsGreen

    Expression

    Mammalian

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBLADE(FP6*)-mCherry

    Plasmid

    #168048

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Inserts

    Blue light-inducible AraC dimers in Escherichia coli
    mCherry
    

    Expression

    Bacterial

    Promoter

    J23101* and PBAD

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pH3mCHSH2

    Plasmid

    #101053

    Purpose

    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

    Depositor

    Jennifer Lodge

    Article

    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

    Insert

    Cryptococcus mCherry expression plasmid
    

    Expression

    Yeast

    Promoter

    Cryptococcus Histone3 promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. ZIKV_Sensor_27B_LacZ

    Plasmid

    #75006

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    Alexander Green, James Collins

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_27B_LacZ
    

    Tags

    LacZ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLNCX2-mEGFP-CHMP2A

    Plasmid

    #115328

    Purpose

    expresses mEGFP-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pInducer20 CARD8-HA PAM

    Plasmid

    #169982

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. VfAU1-LOV_P2A_casp9

    Plasmid

    #131224

    Purpose

    Expresses Ha-tagged VfAU1-LOV and myc-tagged casp9 (domains of Opto-casp9) separated by P2A site

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    VfAU1-LOV-HA_P2A_myc-casp9
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

    Plasmid

    #164140

    Purpose

    AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

    Depositor

    Hajime Hirase, Hirokazu Hirai

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    R-CaMP1.07
    

    Use

    AAV

    Promoter

    hGFAP

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159845

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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30. Opto-casp9_AtCRY2-PHR-casp9

    Plasmid

    #131221

    Purpose

    Expresses Opto-casp9_AtCRY2-PHR-casp9 (light-activated caspase9)

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    Opto-casp9 (AtCRY2-PHR-casp9)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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