We narrowed to 14,028 results for: RING

1. pDx_mScarlet-I3-NCwt-SYFP2

   Plasmid

   #189765

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3-NCwt-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SGLT1(GFP)-MAP17(nb)

   Plasmid

   #216210

   Purpose

   for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

   Depositor

   Lei Chen

   Article

   Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

   Insert

   SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAEL005635-Cas9

   Plasmid

   #100608

   Purpose

   The promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL005635-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Promoter

   AAEL005635 and Opie2

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Equalizer-L episome

   Plasmid

   #169738

   Purpose

   Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2-elavl3-WHaloCaMP1a-EGFP

   Plasmid

   #205311

   Purpose

   Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a-EGFP
   

   Use

   Zebrafish expression

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSET-pr-Kaede (Kaede-A69T)

   Plasmid

   #99228

   Purpose

   Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-Kaede
   

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJJR82

   Plasmid

   #75027

   Purpose

   EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   EGFP^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized EGFP

   Expression

   Worm

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJJR83

   Plasmid

   #75028

   Purpose

   mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   mCherry^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized mCherry

   Expression

   Worm

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-S100A10

   Plasmid

   #107200

   Purpose

   Expresses YFP-human S100A10 fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Zobiack et al J Cell Sci. 2002 Jan 1;115(Pt 1):91-8.

   Insert

   S100A10 (S100A10 Human)
   

   Tags

   Yellow Fluorescent Protein

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-DU422gp140-TM

    Plasmid

    #123251

    Purpose

    Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 isolate with a transmembrane tether

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422) Env
    

    Tags

    CD5 leader peptide and Linker (with 6his tag) and…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pyc111s

    Plasmid

    #158781

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Zachary Knight, Jennifer Garrison

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6s-RPL10a
    

    Use

    AAV

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-haA3A-CBE-G-NG

    Plasmid

    #220897

    Purpose

    Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G-NG
    

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-haA3A-CBE-G

    Plasmid

    #220894

    Purpose

    Express haA3A-CBE-G (with SpCas9) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G
    

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. meffBlue chromoprotein

    Plasmid

    #117845

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMD19_STARR-seq_plants

    Plasmid

    #117379

    Purpose

    STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

    Depositor

    Xiaoyu Zhang

    Article

    Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

    Type

    Empty backbone

    Tags

    GFP derived from pMDC107

    Expression

    Plant

    Promoter

    35s core promoter

    Available Since

    Nov. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCMV-haA3A-CBE-A

    Plasmid

    #220892

    Purpose

    Express haA3A-CBE-A (with SpCas9) in mammalian cells

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-A
    

    Expression

    Mammalian

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO 62.4

    Plasmid

    #123123

    Purpose

    Human expression vector for deltaproteobacteria CasX and sgRNA

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Inserts

    CasX protein
    CasX sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p21 shRNA1

    Plasmid

    #25868

    Depositor

    Sally Temple

    Article

    Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

    Insert

    p21 (Cdkn1a Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-ChRger3-TS-EYFP

    Plasmid

    #127240

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger3-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Promoter

    hSyn1

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-CD63-FKBP-Myc

    Plasmid

    #177910

    Purpose

    human CD63-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD63 (CD63 Human)
    

    Tags

    FKBP and Myc tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBLO 62.5

    Plasmid

    #123124

    Purpose

    Human expression vector for planctomyces CasX and sgRNA

    Depositor

    Jennifer Doudna, Benjamin Oakes

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Inserts

    CasX protein
    CasX sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAGGS-PPP1CB-miRFP703

    Plasmid

    #178524

    Purpose

    Expresses PP1c-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    PP1c-miRFP703 (PPP1CB Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCry2PHR-mCh-RhoA

    Plasmid

    #42958

    Purpose

    Expresses optogenetically controlled RhoA

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pExodus CMV.Trex2

    Plasmid

    #40210

    Purpose

    Mammalian expression of codon-optimized Trex2.

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

    Insert

    Three prime repair exonuclease 2 (Trex2 Mouse)
    

    Expression

    Bacterial and Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pPB9v1

    Plasmid

    #210028

    Purpose

    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAD-LyseR (in lacZ deficient strain)

    Plasmid

    #99245

    Purpose

    For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

    Depositor

    Jeff Hasty

    Article

    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

    Insert

    phage lambda gene R (R phage lambda)
    

    Expression

    Bacterial

    Promoter

    beta-lactamase promoter

    Available Since

    Oct. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pX330a dCas9-VP64

    Plasmid

    #92363

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to VP64 transcriptional activation domain. For targeted gene activation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-MyosinVa-Ctail

    Plasmid

    #110169

    Purpose

    Dominant negative effect by competing with MyosinVa C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Va (Myo5a Mouse)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    amino acids 1444-1853

    Promoter

    CMV

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pFNC-3

    Plasmid

    #233296

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Expression

    Bacterial

    Promoter

    PEM7 and unknown

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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