We narrowed to 17,718 results for: SHE

1. MS2_SC2_S_Eta

   Plasmid

   #175954

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Eta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Eta (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. MS2_SC2_S_Gamma

   Plasmid

   #175047

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Gamma SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Gamma (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. MS2_SC2_S_Theta

   Plasmid

   #175951

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Theta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Theta (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MS2_SC2_S_Zeta

   Plasmid

   #175952

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Zeta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Zeta (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pChd4EosHaloTAF_Puro

   Plasmid

   #172099

   Purpose

   Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion

   Depositor

   Brian Hendrich

   Insert

   chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
   

   Use

   Mouse Targeting

   Tags

   3xFLAG, Avi, Eos, Halo, and TEV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

   Plasmid

   #174139

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   VRQR=D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDM_N1_400

   Plasmid

   #170474

   Purpose

   To amplify transcription template containing LacI operators

   Depositor

   Laura Finzi

   Article

   Xu et al Nucleic Acids Res. 2022 Mar 21;50(5):282

   Insert

   T7A1, O-, O1, Lambda t1 in modified lacZ fragment
   

   Expression

   Bacterial

   Mutation

   diminished hairpins near O1 and diminished latent…

   Promoter

   T7A1

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-loxP-3xpA-loxP-H2B-sfGFP+pCI syntron-T2A-tdTomato+RbG and HsbG syntrons

   Plasmid

   #172480

   Purpose

   H2B-sfGFP with a synthetic intron from the pCI expression vector, T2A, and tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB6-Id1-T2A-tdTomato-FNF-TK

   Plasmid

   #139281

   Purpose

   B6J Id1-T2A-tdTomato allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Id1 (Id1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   C-terminal fusion of T2A-tdTomato

   Expression

   Mammalian

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB6-Gfap-T2A-td-sfGFP-FNF-DTA

    Plasmid

    #137044

    Purpose

    B6J Gfap-T2A-td-sfGFP allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    Gfap (Gfap Mouse)
    

    Use

    Mouse Targeting

    Tags

    T2A-td-sfGFP

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. OA-986C

    Plasmid

    #125000

    Purpose

    Expresses dCas9-VPR driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VPR
    

    Use

    Piggyback

    Expression

    Insect

    Promoter

    bottleneck

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. OA-986D

    Plasmid

    #125001

    Purpose

    Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Expression

    Insect

    Promoter

    ubiquitin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA-986E

    Plasmid

    #125002

    Purpose

    Expresses dCas9-VP64 driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Expression

    Insect

    Promoter

    bottleneck

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pANTO3

    Plasmid

    #131107

    Purpose

    To generate a replicative plasmid carrying mycobacteriophage L5 integrase

    Depositor

    Riccardo Manganelli

    Article

    Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

    Insert

    mycobacteriophage L5 integrase
    

    Expression

    Bacterial

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ad-mMKP3 S159A S197A-Flag

    Plasmid

    #131579

    Purpose

    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Mutation

    changed Serine 159 to Alanine and Serine 197 to A…

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFGRNA-LbCpf1-SpCas9

    Plasmid

    #119673

    Purpose

    Empty vector for cloning LbCpf1-SpCas9 fgRNA

    Depositor

    Yongsub Kim

    Article

    Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFGRNA-AsCpf1-SpCas9

    Plasmid

    #119674

    Purpose

    Empty vector for cloning AsCpf1-SpCas9 fgRNA

    Depositor

    Yongsub Kim

    Article

    Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFGRNA-LbCpf1-spCas9-MS2

    Plasmid

    #119675

    Purpose

    Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

    Depositor

    Yongsub Kim

    Article

    Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSM41

    Plasmid

    #113940

    Purpose

    Constitutive bacterial expression of red fluorescent Ca2+ sensor REX-GECO1

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    REX-GECO1
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Myc-gamma1 (in pCAGGS)

    Plasmid

    #118039

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

    Plasmid

    #114728

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M5
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBABE.zeo/hKGA.S286A

    Plasmid

    #110357

    Purpose

    Mammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tag

    Depositor

    Sandra Martha Gomes Dias

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S286A

    Promoter

    gag

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTorPE-Y-GECO2m

    Plasmid

    #105066

    Purpose

    A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2m
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. His6-Halo-TEV-Akt2

    Plasmid

    #87606

    Purpose

    expresses His-halo tagged at the N-terminus of human Akt2 protein

    Depositor

    Yimon Aye

    Insert

    AKT 2 (AKT2 Human)
    

    Use

    Zebrafish (danio rerio) expression

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. Hs.KRAS4b M72C

    Plasmid

    #83160

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M72C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    M72C

    Available Since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. Hs.KRAS4b R73K

    Plasmid

    #83163

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73K mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    R73K

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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27. Hs.KRAS4b R73E

    Plasmid

    #83162

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), R73E mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    R73E

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. Hs.KRAS4b Y40F

    Plasmid

    #83161

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y40F mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Y40F

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. Hs.KRAS4b Y40C

    Plasmid

    #83159

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Y40C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Y40C

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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30. Hs.KRAS4b E37G

    Plasmid

    #83158

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), E37G mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    E37G

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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