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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,718 results for: SHE

Showing: 10051 - 10080 of 17718 results

  1. HaloTag-LacI-mHP1a

    Plasmid
    #232637
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-mHP1a (Cbx5 Synthetic, Mouse)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232637-plasmid-map-sequence-id-463625

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  4. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229851-plasmid-map-sequence-id-460711

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  8. Galt1-HT7-mGold

    Plasmid
    #230027
    Purpose
    Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-HaloTag7-mGold (B4GALT1 Synthetic, Human)
    Tags
    HaloTag,mGold
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230027-plasmid-map-sequence-id-458169

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  9. pCAGGS-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222016
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222016-plasmid-map-sequence-id-457428

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  10. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222017
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    deleted stop codon (TAA)
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222017-plasmid-map-sequence-id-457152

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  11. AICSDP-167:CDH5-mEGFP

    Plasmid
    #217425
    Purpose
    Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5
    Depositor
    Allen Institute for Cell Science
    Insert
    CDH5 Homology Arms with mEGFP (CDH5 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217425-plasmid-map-sequence-id-454212

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  13. pSN667

    Plasmid
    #219963
    Purpose
    Expressed sfGFP fused human KIF1Bß(1-721) in insect cells
    Depositor
    Shinsuke Niwa
    Insert
    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    Tags
    sfGFP::2xStrepII tag
    Expression
    Insect
    Mutation
    encodes 1-721
    Promoter
    polyhedrin
    Available Since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    219963-plasmid-map-sequence-id-441237

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  14. MARS-nGFP

    Plasmid
    #205234
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205234-plasmid-map-sequence-id-404747

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  15. pCDNA3-2xMARS-PLD

    Plasmid
    #205237
    Purpose
    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
    Depositor
    Jeremy Baskin
    Inserts
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205237-plasmid-map-sequence-id-404742

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  16. pSN668

    Plasmid
    #219962
    Purpose
    Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells
    Depositor
    Shinsuke Niwa
    Insert
    codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
    Tags
    sfGFP::2xStrepII tag
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219962-plasmid-map-sequence-id-440846

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  17. pLV-SFFV-ZFYVE27-WPRE-UbC-Emerald

    Plasmid
    #214469
    Purpose
    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    214469-plasmid-map-sequence-id-425107

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  20. pAG426-GAL-APP-CTF

    Plasmid
    #181732
    Purpose
    Galactose inducible expression of APP-CTF
    Depositor
    Alejandro Chavez
    Insert
    APP-CTF (APP Human)
    Expression
    Yeast
    Mutation
    C-terminal fragment (aa 671-770)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    181732-plasmid-map-sequence-id-437192

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  21. PE-SUMO CRYGS Q16C

    Plasmid
    #217674
    Purpose
    For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine
    Promoter
    T7
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    217674-plasmid-map-sequence-id-434908

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  22. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  23. pAAV/CMV-SCLM

    Plasmid
    #216758
    Purpose
    AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon followed by WPRE and human growth hormone polyA terminator
    Use
    AAV
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CMV (cytomegalovirus)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216758-plasmid-map-sequence-id-429489

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Showing: 10051 - 10080 of 17718 results