We narrowed to 5,233 results for: phy
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Plasmid#116016Purposedestination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-inductionDepositorInsertpOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
ExpressionPlantAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
Plasmid#65662PurposeFLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)
Plasmid#174639PurposeOptogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)DepositorInsertSSPB(micro)-GFP-ppKinVIb(861-1321)
TagsSSPB(micro)-GFPExpressionMammalianMutationSSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…PromoterChicken beta-actinAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPKm-293
Plasmid#105817PurposepcDNA - TET DBD - MTAD. Expresses TET DNA binding domain fused to MTADDepositorInsertTET DBD and MTAD
ExpressionMammalianPromoterCMVAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP1 R152Q GFP
Plasmid#51884Purposeexpression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 R152Q (tpte2 Frog)
UseXenopus oocyte expressionTagsEGFPExpressionMammalianMutationR152Q: near the extracellular side of the fourth …PromoterCMVAvailable SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlluciferase
Plasmid#63853PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyPromotertacAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTNos:BASTA:PNos (GB0023)
Plasmid#68211PurposeProvides the bar gene for resistance to basta fused to the A. tumefaciens Nopaline synthase promoter and terminator. Cloned as a level 0 GB part ready to be transferred to a GB level1 plant expression vector.DepositorInsertbar gene
UseUnspecifiedMutationBsaI and BsmBI sites removedPromoterPNosAvailable SinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable SinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19-NLS-4
Plasmid#54222PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorAvailable SinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits