We narrowed to 5,233 results for: phy
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Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35S::AtSOBIR1-YFPn
Plasmid#86165PurposeSOBIR1 split YFPDepositorAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLexA::SRDX (GB0732)
Plasmid#68184PurposeProvides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLexA Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD::BDR (GB0734)
Plasmid#68185PurposeProvides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertGal4 Binding Domain fused to BDR Transcriptional Repressor
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR
Plasmid#65091PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged sequence to render plasmid resistant to s…PromoterCMV, 2x TetAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_K36E_R38E
Plasmid#65095PurposeDestination vector with RBPMS with K36E/R38E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchange lysine 36 to glutamic acid and arginine 38…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only