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pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101111

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to AVPR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

AVPR2-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

Plasmid

#180273

Purpose

Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Plasmid

#107311

Purpose

Encoding scFv-p65-HSF1 driven by EF1α promoter

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSC014_pLKO-U6-BsmBI-MS2sgRNA-EFS-Thy11-2A-MS2-p65-HSF1

Plasmid

#192187

Purpose

TdgA vector

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCA24-pHR-CMV-stdMCP-p65-HSF1-T2A-GFP

Plasmid

#199455

Purpose

Expression of stdMCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdMCP-PH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCA25-pHR-CMV-stdPCP-p65-HSF1-T2A-GFP

Plasmid

#199456

Purpose

Expression of stdPCP-PH in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

stdPCP-PH

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180266

Purpose

Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

No-Target Control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180267

Purpose

Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

EGFR sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pZR047_Lenti-U6-sgGARP-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

Plasmid

#180268

Purpose

Lentiviral expression vector for CRISPRa-SAM with GARP targeting sgRNA

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

GARP sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR

Plasmid

#186744

Purpose

EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backbone

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP

Use

Lentiviral

Tags

P2A-BFP

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA

Plasmid

#107307

Purpose

Encoding an SPH activation system

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

CAG-LSL-dCas9-10xGCN4-P2A-scFv-P65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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H1DBD-Halo-HA

Plasmid

#200203

Purpose

Expression of truncated HSF1 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H1DBD-Halo-HA (Hsf1 Mouse)

Use

Tags

Halo; HA

Expression

Mammalian

Mutation

HSF1 (1-399)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H1DBD-HR-Halo

Plasmid

#200204

Purpose

Expression of truncated HSF1 (DBD and HR)

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H1DBD-HR-Halo (Hsf1 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-672)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-H1DBD-H2

Plasmid

#200205

Purpose

Expression of HSF2 with HSF1 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H1DBD-H2 (Hsf1, Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-399); HSF2 (366-1554)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
H2DBD-H1-Halo

Plasmid

#200193

Purpose

Expression of HSF1 with HSF2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H2DBD-H1-Halo (Hsf1, Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366); HSF1 (399-1576)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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lentiMPH v2 (Neo)

Plasmid

#92065

Purpose

Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Insert

MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a-MVPH

Plasmid

#235594

Purpose

Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

MS2-VP64-P65-HSF1

Use

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-MPH

Plasmid

#235596

Purpose

Expresses the MS2-P65-HSF1 under the control of human EF1a promoter

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

MS2-P65-HSF1

Use

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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