We narrowed to 259 results for: kevin janes

1. pEN_TTmiRc2_BirA_CDKN1A

   Plasmid

   #136523

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   CDKN1A (CDKN1A Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEN_TTmiRc2_BirA

   Plasmid

   #136521

   Purpose

   Used as a donor vector to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   BirA
   

   Use

   Entry vector for gateway cloning

   Tags

   Expression

   Mutation

   R118G (highly promiscuous form)

   Promoter

   Available since

   June 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLIK TT 3xFLAG LacZ hygro

   Plasmid

   #98395

   Purpose

   Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEN_TT miRc2 3xFLAG

   Plasmid

   #83274

   Purpose

   Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Type

   Empty backbone

   Use

   Entry vector for gateway cloning

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   TRE tight

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEN_TTmiRC2_3xflag_NRF2(RR)

   Plasmid

   #136522

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   This plasmid is developed by mutating 3 base pair…

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN_TT MEK5-DD-mRFP1

   Plasmid

   #47547

   Purpose

   Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

   Insert

   MEK5-DD (MAP2K5 Human)
   

   Use

   Entry vector

   Tags

   mRFP

   Expression

   Mutation

   S311D, T315D

   Promoter

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pEN_TTmiRC2_p53DD

   Plasmid

   #136520

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53DD (Trp53 Mouse)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Expression

   Mutation

   This construct was created by PCR from the pBabe …

   Promoter

   Available since

   Oct. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe 3XFLAG-S37A-GATA6-3XAU1 puro

   Plasmid

   #72608

   Purpose

   For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Mutation

   changed serine37 to alanine

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

9. TET-pLKO.1 PURO shGDF11 #1

   Plasmid

   #83083

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shGDF11 (GDF11, Gdf11 Human, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pLKO.1 shPIK3CD.v2 puro

    Plasmid

    #58707

    Purpose

    Lentiviral shRNA vector for knockdown of human PIK3CD

    Depositor

    Kevin Janes

    Article

    Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Insert

    shPIK3CD (PIK3CD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pSLIK 3XFLAG-MKK7-EE-NLS hygro

    Plasmid

    #87780

    Purpose

    Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7 (Map2k7 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

12. tet-pLKO shMKP7.v1 puro

    Plasmid

    #87794

    Purpose

    Expresses an inducible short hairpin targeting human MKP7 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP7 (DUSP16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tet-pLKO shDUSP19 puro

    Plasmid

    #87796

    Purpose

    Expresses an inducible short hairpin targeting human DUSP19 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shDUSP19 (DUSP19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. shMEK1/2 neo

    Plasmid

    #72569

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN_TT 3xHA-MKK6-EE

    Plasmid

    #47544

    Purpose

    Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    3xHA-MKK6-EE (MAP2K6 Human)
    

    Use

    Entry vector

    Tags

    3XHA

    Expression

    Mutation

    S207E, T211E

    Promoter

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pDual_dsCas9_Puro

    Plasmid

    #214680

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid

    #214682

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid

    #214683

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDual_dsCas9_Venus_hs_NQO1

    Plasmid

    #214684

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human NQO1

    Depositor

    Kevin Janes

    Insert

    dgRNA_NQO1 (NQO1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDual_dsCas9_Venus_hs_TP73

    Plasmid

    #214685

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid

    #214686

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDual_dsCas9_BFP_hs_TP73

    Plasmid

    #214687

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid

    #214688

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Puromycin resistance cassette has silent mutation…

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDual_dsCas9_Puro_hs_TP73

    Plasmid

    #214689

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73

    Depositor

    Kevin Janes

    Insert

    dgRNA_TP73 (TP73 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Puromycin resistance cassette has silent mutation…

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDual_dsCas9_Venus_hs_NRF2

    Plasmid

    #214692

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human NRF2

    Depositor

    Kevin Janes

    Insert

    dgRNA_NRF2 (NFE2L2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDual_dsCas9_Venus

    Plasmid

    #214678

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEN_TT luciferase-V5

    Plasmid

    #220360

    Purpose

    Gateway entry vector for an inducible V5-tagged luciferase

    Depositor

    Kevin Janes

    Insert

    luciferase
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEN_TT DNM1L-V5

    Plasmid

    #220359

    Purpose

    Gateway entry vector for an inducible V5-tagged DNM1L

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSLIK DNM1L-V5 neo

    Plasmid

    #220363

    Purpose

    Lentiviral expression vector for an inducible V5-tagged DNM1L construct

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLIK luciferase-V5 neo

    Plasmid

    #220364

    Purpose

    Lentiviral expression vector for an inducible V5-tagged luciferase construct

    Depositor

    Kevin Janes

    Insert

    luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge