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We narrowed to 6,722 results for: tac

Showing: 3001 - 3050 of 6722 results

  17. pDIRECT_26H

    Plasmid
    #91150
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91150-plasmid-map-sequence-id-182284

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  18. pDONR P2R-P3-mKate2

    Plasmid
    #48345
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a stop codon at the 3' end of the c…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48345-plasmid-map-sequence-id-70403

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  19. PM2-FRB-CFP

    Plasmid
    #67518
    Purpose
    FRB conjugated plasma membrane targeting sequences 2 from Lyn11(GCIKSKGKDSA)
    Depositor
    Tamas Balla
    Article
    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.
    Insert
    mTOR (MTOR Human)
    Use
    Tags
    CFP, HA, and PM tethering sequence from human Lyn…
    Expression
    Mammalian
    Mutation
    FRB domain only (residues 2019–2114)
    Promoter
    CMV
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    68709-plasmid-map-sequence-id-125636

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  23. pPEE46

    Plasmid
    #139425
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mRuby3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    139425-plasmid-map-sequence-id-292473

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  24. pPyCAG-NE-mKate2-IRES-Hph

    Plasmid
    #183616
    Purpose
    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.
    Depositor
    Sally Lowell
    Article
    Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do
    Insert
    mKate2
    Use
    Tags
    Human EMD transmembrane domain and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183616-plasmid-map-sequence-id-361612

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  35. Arch(D95H)-eGFP

    Plasmid
    #51081
    Purpose
    Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Histidine
    Promoter
    Ubiquitin
    Available since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51081-plasmid-map-sequence-id-77349

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  36. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

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  40. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91149-plasmid-map-sequence-id-182283

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  44. pDIRECT_25G

    Plasmid
    #91144
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91144-plasmid-map-sequence-id-182272

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  47. pGEM-PH-citrine

    Plasmid
    #131406
    Purpose
    Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrine
    Depositor
    Lars Hufnagel
    Article
    Guignard et al Science. 2020 Jul 10;369(6500). pii: 369
    Insert
    PH
    Use
    In vitro transcription
    Tags
    citrine yellow fluorescent protein
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131406-plasmid-map-sequence-id-261478

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  49. pDIRECT_25I

    Plasmid
    #91146
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91146-plasmid-map-sequence-id-182476

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Showing: 3001 - 3050 of 6722 results