We narrowed to 611 results for: vp64

1. pHRm-GFP(1-10)-VP64-NLS

   Plasmid

   #70228

   Purpose

   Expresses GFP(1-10)-VP64-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP(1-10)-VP64-NLS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-dCas9-VP64-6xHis

   Plasmid

   #62935

   Purpose

   Expression of dCas9-VP64-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   dCas9-VP64
   

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A and H840A amino acid changes render Cas9 nuc…

   Promoter

   T7

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-scFvGCN4sfGFP-VP64-GB1

   Plasmid

   #141417

   Purpose

   Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation

   Depositor

   Izuho Hatada

   Article

   Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i

   Insert

   scFvGCN4sfGFP-VP64-GB1
   

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE TRE dCas9-VP64

   Plasmid

   #50916

   Purpose

   Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A nuclease-deficient

   Promoter

   TRE

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE EF1α dCas9-VP64

   Plasmid

   #50918

   Purpose

   Constitutive dCas9-VP64 lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5aa SunTag VP64 EVD

   Plasmid

   #115483

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. hNFAT2-409:TALE:VP64:KRɸ

   Plasmid

   #160661

   Purpose

   Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   hNFAT2-409:TALE:VP64:KRɸ
   

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5aa SunTag VP64 CLV3

    Plasmid

    #115485

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5aa SunTag VP64 AP3

    Plasmid

    #115484

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the AP3 locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Gal4DB-NS3-VP64-p65_IRES_mCherry

    Plasmid

    #112627

    Purpose

    Encodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    Gal4DB-NS3-VP64-p65
    

    Tags

    AU1 (N term on NS3), HA (C term on NS3), and T7 (…

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mNFAT4-462:TALE:VP64:KRɸ

    Plasmid

    #160662

    Purpose

    Constitutive expression of murine NFAT (aa 4-462) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    mNFAT4-462:TALE:VP64:KRɸ
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. rTetR-NS3-VP64-p65_IRES_mCherry

    Plasmid

    #112628

    Purpose

    Encodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    rTetR-NS3-VP64-p65
    

    Tags

    AU1 (N term on NS3), HA (C term on NS3), and T7 (…

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-EF1alpha-scFv-VP64_Blast

    Plasmid

    #192655

    Purpose

    3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-VP64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416-Gal4-dCas9-VP64

    Plasmid

    #71128

    Purpose

    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

    Depositor

    Ronald Davis

    Article

    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

    Insert

    Gal4-dCas9-VP64
    

    Tags

    NLS n-terminal, N terminal Gal4 Activator domain,…

    Expression

    Yeast

    Promoter

    Tef1

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

    Plasmid

    #203911

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C2]-VP64
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-dHeFSpCas9-VP64-6xHis

    Plasmid

    #92119

    Purpose

    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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