Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 1,571 results for: Tef;

Showing: 281 - 320 of 1571 results

  1. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

    Enlarge

  2. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

    Enlarge

  6. TFORF2693

    Plasmid
    #142359
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD4 (TEAD4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142359-plasmid-map-sequence-id-397176

    Enlarge

  12. pJT1

    Plasmid
    #98299
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    98299-plasmid-map-sequence-id-189966

    Enlarge

  13. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Promoter
    Available since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98306-plasmid-map-sequence-id-181809

    Enlarge

  15. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Article
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127546-plasmid-map-sequence-id-251803

    Enlarge

  16. TFORF2692

    Plasmid
    #142358
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD1 (TEAD1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142358-plasmid-map-sequence-id-397200

    Enlarge

  17. TFORF2688

    Plasmid
    #143374
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD3 (TEAD3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    143374-plasmid-map-sequence-id-392749

    Enlarge

  18. TFORF2689

    Plasmid
    #144148
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD2 (TEAD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144148-plasmid-map-sequence-id-396213

    Enlarge

  19. TFORF2690

    Plasmid
    #144149
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD2 (TEAD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144149-plasmid-map-sequence-id-392336

    Enlarge

  20. TFORF2691

    Plasmid
    #142357
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TEAD2 (TEAD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142357-plasmid-map-sequence-id-397142

    Enlarge

  21. pILFPQ13A7

    Plasmid
    #223837
    Purpose
    Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Insert
    pTEF1>mScarletI3>
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223837-plasmid-map-sequence-id-446736

    Enlarge

  22. pILFPQ13B7

    Plasmid
    #223838
    Purpose
    Expressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Insert
    pTEF1>StayGold>
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223838-plasmid-map-sequence-id-446738

    Enlarge

  23. pILFPmTagBFP2FrFAST

    Plasmid
    #223839
    Purpose
    Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae
    Depositor
    Claudia Vickers
    Insert
    pTEF1>mTagBFP2>FrFAST
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223839-plasmid-map-sequence-id-455093

    Enlarge

  24. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. pTXB3-Flag PLK1 T210D

    Plasmid
    #68272
    Purpose
    bacterial expression of Plk1 T210D mutant
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Use
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    T210D
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pILGFP3A2 (2)

    Plasmid
    #185852
    Purpose
    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    185852-plasmid-map-sequence-id-370761

    Enlarge

  40. pILGFP9E3

    Plasmid
    #185842
    Purpose
    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185842-plasmid-map-sequence-id-368107

    Enlarge

Showing: 281 - 320 of 1571 results