We narrowed to 5,620 results for: pcas

1. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

   Plasmid

   #108773

   Purpose

   Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   VCre recombinase fragment N-terminus to aa269
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GID1,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

   Plasmid

   #108774

   Purpose

   Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   VCre recombinase fragment aa270 to C-terminus
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   GAI and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

   Plasmid

   #108840

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA

   Plasmid

   #108836

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FRB,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA

   Plasmid

   #108842

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GAI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

   Plasmid

   #178996

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

   Plasmid

   #178995

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

   Plasmid

   #128167

   Purpose

   Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

   Plasmid

   #107323

   Purpose

   Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-NmCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)

    Plasmid

    #36879

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

    Plasmid

    #182049

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A/T258A

    Promoter

    CAG

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA

    Plasmid

    #108831

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA

    Plasmid

    #108838

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    PYL,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA

    Plasmid

    #108834

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FKBP,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108835

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpA

    Plasmid

    #108823

    Purpose

    Expresses N-terminus to aa270 of Cre recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa270
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

    Plasmid

    #75243

    Purpose

    CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA

    Plasmid

    #108738

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to FKBP CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FKBP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

    Plasmid

    #69224

    Purpose

    Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS and Zif268

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpA

    Plasmid

    #108783

    Purpose

    Expresses N-terminus to aa172 of VCre recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa172
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBW2587_pCAG-PV1-PYL-L1-VCre-173C-NLS-BGHpA

    Plasmid

    #108784

    Purpose

    Expresses aa173 to C-terminus of VCre recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa173 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA

    Plasmid

    #108813

    Purpose

    Expresses N-terminus to aa326 of TP901 integrase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    TP901 integrase fragment N-terminus to aa326
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA

    Plasmid

    #108801

    Purpose

    Expresses N-terminus to aa428 of PhiC31 integrase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa428
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA

    Plasmid

    #108765

    Purpose

    Expresses N-terminus to aa168 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa168
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA

    Plasmid

    #108766

    Purpose

    Expresses aa169 to C-terminus of Flp recombinase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment aa169 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA

    Plasmid

    #108727

    Purpose

    Expresses N-terminus to aa256 of Cre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa256
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA

    Plasmid

    #108728

    Purpose

    Expresses aa257 to C-terminus of Cre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa257 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA

    Plasmid

    #108817

    Purpose

    Expresses N-terminus to aa229 of Cre recombinase fused to nMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa229
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge