Addgene

Showing: 21 - 40 of 948 results

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Mutant Human Cortactin-GFP

Plasmid

#71365

Purpose

mammalian expression of mutant human Cortactin-GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

cortactin (CTTN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

silent mutation present at 72nd nucleotide

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YF EGFP

Plasmid

#187263

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

3YF (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YD EGFP

Plasmid

#187264

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Availability

Academic Institutions and Nonprofits only

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GFP-alpha-E-Catenin 3xFP4 (895)

Plasmid

#67936

Purpose

Mammalian expression of alpha E catenin

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

alpha E catenin (Ctnna1 Mouse)

Use

Tags

3xFP4 and EGFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
MRLC-DD-pIRES-EGFP-AP4-mito

Plasmid

#101287

Purpose

To define the role of Mena/VASP in tension-sensitive actin assembly

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

MRLC-DD and AP4-Mito

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinFL

Plasmid

#187272

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

U6, CMV

Availability

Academic Institutions and Nonprofits only

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GFP-mouse vinculin full length (889)

Plasmid

#67935

Purpose

Mammalian expression of mouse vinculin with GFP fusion

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

Vinculin (Vcl Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L159P in vinculin

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

Plasmid

#187274

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Anillin A740D, E758K

Promoter

U6, 5'LTR

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

Plasmid

#101285

Purpose

Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-H-Ras

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H-Ras V12

Promoter

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha2 EGFP-N3

Plasmid

#13601

Depositor

Dean Sheppard

Insert

integrin alpha 9 / alpha 2 (ITGA9 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
integrin alpha9 / alpha4 EGFP-N3

Plasmid

#13602

Depositor

Dean Sheppard

Insert

integrin alpha 9 / alpha 4 (ITGA9 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Promoter

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 / alpha5 EGFP-N3

Plasmid

#13603

Depositor

Dean Sheppard

Insert

integrin alpha 9 / alpha 5 (ITGA9 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
integrin alpha9 / alpha2 EGFP pBabe puro

Plasmid

#13606

Depositor

Dean Sheppard

Insert

integrin alpha 9 / alpha 2 (ITGA9 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Promoter

Availability

Academic Institutions and Nonprofits only
integrin alpha9 / alpha5 EGFP pBabe puro

Plasmid

#13608

Depositor

Dean Sheppard

Insert

integrin alpha 9 / alpha 5 (ITGA9 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Integrin alpha 9 chimera containing the cytoplasm…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-p38alpha

Plasmid

#86832

Purpose

mammalian expression of GFP-p38alpha

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38alpha (MAPK14 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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GFP-HP1alpha

Plasmid

#17652

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 alpha (CBX5 Human)

Use

Tags

GFP and His

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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GFP-TNFalpha

Plasmid

#28089

Depositor

Jennifer Stow

Article

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

TNFalpha (Tnf Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
palphaGGFPR

Plasmid

#64377

Purpose

Alpha-gliadin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

alpha-Gliadin promoter

Use

Tags

GFP

Expression

Plant

Mutation

Promoter

alpha-Gliadin

Availability

Academic Institutions and Nonprofits only
pDGB1alpha2_KanR_BastaR_Rb7_GFP

Plasmid

#186427

Purpose

optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar) with fluorescent marker (eGFP)

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Inserts

KanR
BastaR
Rb7
eGFP

Use

Synthetic Biology; Binary vector for escherichia …

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

CaMV 35S and Pnos

Availability

Academic Institutions and Nonprofits only

Enlarge