1,775 results

1. pSEM254 - sgRNA mosTI neoR

   Plasmid

   #159831

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI neoR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM322 - array co-injection fragment mosTI neoR

   Plasmid

   #159832

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   array co-injection fragment mosTI neoR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDD104 (Peft-3::Cre)

   Plasmid

   #47551

   Purpose

   Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Insert

   Cre Recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

   Plasmid

   #159899

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | tagRFP | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPK605

   Plasmid

   #38148

   Depositor

   Patricia Kuwabara

   Inserts

   unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
   pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   pie-1 promoter and unc-119

   Available Since

   Oct. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW2098

   Plasmid

   #163094

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Promoter

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF11-wElectra2

   Plasmid

   #184933

   Purpose

   Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   tag-168

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSF143-wArchon1-KGC-EGFP-ER2

   Plasmid

   #108429

   Purpose

   Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   wArchon1-KGC-EGFP-ER2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   flp-18

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNMSB104

   Plasmid

   #199314

   Purpose

   MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

   Depositor

   Michael Krieg

   Article

   Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

   Insert

   flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChRmine::SL2::jRGECO1a::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   mTagBFP2 from pJJR81, C. elegans codon optimized …

   Promoter

   flp-18

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJR031

    Plasmid

    #198825

    Purpose

    Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD287 (mKate2^SEC^3xMyc

    Plasmid

    #70685

    Purpose

    mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xMyc and C. elegans codon-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSF11-wmTagBFP2

    Plasmid

    #184934

    Purpose

    Codon optimized blue fluorescent protein mTagBFP2 in C.elegans expresison plasmid

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF11-wmiRFP713-T2A-HO1

    Plasmid

    #197246

    Purpose

    Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP713-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEZ-1

    Plasmid

    #51868

    Purpose

    Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

    Insert

    psdhb-1::mtLS-cpYFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    sdhb-1

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

    Plasmid

    #187100

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-SF-iGluSnFR
    

    Use

    Tags

    mCherry-SL2

    Expression

    Worm

    Mutation

    Promoter

    Ttx-3

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK-EGFP-FKBPF36V

    Plasmid

    #199765

    Purpose

    plasmid for targeting dTAG into the AAVS1 locus

    Depositor

    Hannes Lans

    Article

    van der Woude et al Nucleic Acids Res. 2023 Oct 13;51(18):e9

    Insert

    GFP-dTAG
    

    Use

    Tags

    GFP and dTAG

    Expression

    Mammalian and Worm

    Mutation

    Promoter

    hPGK

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHIP_RNA1

    Plasmid

    #61259

    Purpose

    Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    heat shock promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSF11-wmCardinal

    Plasmid

    #197251

    Purpose

    Expresses the protein of wmCardinal in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmCardinal
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHIP_RNA2FLAG

    Plasmid

    #61261

    Purpose

    Flag tagged Orsay virus RNA2 genome segment expression plasmid

    Depositor

    David Wang

    Article

    Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

    Insert

    Orsay RNA2
    

    Use

    Tags

    Flag fused

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge