Showing: 121 - 140 of 142471 results
-
Plasmid#60445PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKAR
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-FLIM-AKART391A
Plasmid#60446PurposeExpresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKART391A
UseAAV and Cre/LoxTagsExpressionMammalianMutationchanged Threonine 391 to AnaninePromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
dRT-pMDLg/pRRE
Plasmid#60488Purposegag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)DepositorInsertHIV-1 gag, HIV-1 pol with inactive reverse transcriptase
UseTagsExpressionMutationchanged Asp at position 249 + 250 to ValPromoterAvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG2-wPRE-pA
Plasmid#60489PurposeeGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)DepositorInsertIRES-eGFP; wPRE; BGH-pA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSynSRE-Mut-T-Luc
Plasmid#60490PurposeContains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988DepositorInsertHMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
UseLuciferaseTagsLuciferase reporterExpressionMammalianMutationFour point mutations (G->C or A->C) in the …PromoterPartial HMG-CoA synthase promoter (-324/-225) fus…AvailabilityAcademic Institutions and Nonprofits only -
pH2B-SCFP1-2A-mTq2-NES
Plasmid#60491PurposeCo-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasmDepositorInsertsH2B-SCFP1
mTurquoise2-NES
UseTagsSCFP1 and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES
Plasmid#60492PurposeCo-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasmDepositorInserts3xnls-SGFP2(T65G)
SGFP2-3xNES
UseTagsSGFP2 and SGFP2(T65G)ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmVenus(L68V)-mTurquoise2
Plasmid#60493PurposeProduces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)DepositorInsertSYFP2-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-T2A-Venus(L68V)
Plasmid#60494PurposeProduces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.DepositorInsertmTurquoise2-T2A-mVenus(L68V)
UseTagsT2A peptideExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GP
Plasmid#60495PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BP
Plasmid#60496PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RP
Plasmid#60497PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GH
Plasmid#60498PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BH
Plasmid#60499PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RH
Plasmid#60500PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GN
Plasmid#60501PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BN
Plasmid#60502PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RN
Plasmid#60503PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GB
Plasmid#60504PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 142471 results