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Plasmid#116936PurposeYeast integrative vector for the expression of 2xGFPDepositorInsert2xGFP
UseTagsExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EGFP
Plasmid#166140PurposeEGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorArticleInsertEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
3396 pBM272 Bax
Plasmid#8770DepositorInsertBax (Bax Mouse)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3
Plasmid#188064PurposePROSS-designed high-redox potential laccase from Trametes hirsutaDepositorInsertPROSS-designed high-redox potential laccase from Trametes hirsuta
UseTagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 mutations, described in publicationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Tv9nL
Plasmid#188069PurposePROSS-designed high-redox potential laccase from Trametes versicolorDepositorInsertPROSS-designed high-redox potential laccase from Trametes versicolor
UseTagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation79 PROSS mutations, described in publicationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA (V5 and HA)
Plasmid#222485PurposeCo-expression of two genes in yeast with V5 and HA tags and URA3 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSB-NAT
Plasmid#166698PurposeCombined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymNeongreen
Plasmid#193959PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeastDepositorInsertymNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNH605-HSE-YFP
Plasmid#172131PurposeFluorescent reporter for Hsf1 activityDepositorInsert4xHSE-YFP
UseVector for genomic integration into the yeast gen…TagsExpressionYeastMutationPromoterCYC1 core promotor with 4 tandem repeats of the H…Available sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-GW
Plasmid#61703PurposeGateway compatible Y2H vector - BDDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TRP (V5 and HA)
Plasmid#222488PurposeCo-expression of two genes in yeast with V5 and HA tags and TRP1 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS646
Plasmid#183175PurposeY2H vector compatible with pJS647 PYR1 insertDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYDS2.0_AT118-H
Plasmid#222846PurposeYeast-display plasmid encoding Nb. AT118-H, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)DepositorInsertAT118-H
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03
Plasmid#28003DepositorInsertAT1.03
UseTagsmVenus and mseCFPExpressionYeastMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38
Plasmid#65516PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edCit-attR1 and attR2-edCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAS
Plasmid#60847PurposeExpresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeastDepositorInsertsS. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA
UseCRISPR and Synthetic BiologyTagsNLS/His8 and TTT 3' extension prior to sgRNAExpressionBacterial and YeastMutationL4 is UUCG tetraloop and guide targets LYP1 (CATA…PromoterAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only