Showing: 121 - 140 of 141553 results
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Plasmid#52679PurposeshRNA against α-actinin-4 with eGFP transfection markerDepositorInsertACTN4 (Actn4 Rat)
UseRNAiTagsCMV-EGFPExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
UseTagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Snf1-cat
Plasmid#52683Purposeexpression of the Snf1 kinase domain in bacteriaDepositorInsertSNF1 (SNF1 Budding Yeast)
UseTagsGSTExpressionBacterialMutationencodes kinase domain only, aa 41-315PromotertacAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Abl-His6-FLAG
Plasmid#52684Purposeexpression of Abl in mammalian cellsDepositorInsertABL1 (Abl1 Mouse)
UseTagsFLAG and His6ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-LINKER
Plasmid#52685PurposeSame as 41023, with additional linkerDepositorInserttetO7-Pcyc1+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER
Plasmid#52686PurposeSame as 41024, with additional linkerDepositorInserttetO7-Pcyc1-3xHA+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER
Plasmid#52687PurposeSame as 41025, with additional linkerDepositorInserttetO7-Pcyc1-GFP+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GST-LINKER
Plasmid#52688PurposeSame as 41026, with additional linkerDepositorInserttetO7-Pcyc1-GST+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-3xFLAG-LINKER
Plasmid#52689PurposeSame as 41022, with additional linkerDepositorInserttetO7-Pcyc1-3xFLAG+LINKER
UsePromoter replacementTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pREP41-MCS+
Plasmid#52690PurposeSame as pREP41, with better MCSDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPnmt41AvailabilityAcademic Institutions and Nonprofits only -
pREP42-MCS+
Plasmid#52691PurposeSame as pREP42, with better MCSDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterPnmt41AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX-(3×FLAG)-TEV-ProtA
Plasmid#52692PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromotern/aAvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX-(3×FLAG)-TEV-ProtA
Plasmid#52693PurposeFor C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtADepositorInsert(3×FLAG)-TEV-ProtA
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-LIN-41
Plasmid#52716Purposeretroviral expression of human LIN-41 / TRIM71DepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXS-hs-3xHA-LIN-41
Plasmid#52717Purposeretroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaRING-LIN-41
Plasmid#52718Purposeretroviral expression of human LIN-41 / TRIM71 containing a RING domain deletion and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 12-91PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaBBox-LIN-41
Plasmid#52719Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 194-320PromoterAvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaCoiledCoil-LIN-41
Plasmid#52720Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the Coiled Coil domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 328–447PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 141553 results